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Product name
Anti-c-Myc (phospho T358) antibody
See all c-Myc products (9) ...
Description
Rabbit polyclonal to c-Myc (phospho T358)
Specificity
ab30642 detects endogenous levels of Myc only when phosphorylated at Threonine 358.
Tested applications
IHC-P, ELISA, IPmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic phosphopeptide derived from human c-Myc around the phosphorylation site of Threonine 358.
Positive control
Breast carcinoma.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg++ and Ca++), 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Tumor biomarkers >> Oncoproteins
Cancer >> Oncoproteins/suppressors >> Oncoproteins >> Transcription factors
Cancer >> Cell cycle >> Cell differentiation
Epigenetics and Nuclear Signaling >> Transcription >> Transcription Factors
Stem Cells >> Signaling Pathways >> TGF beta >> Nuclear
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> HLH / Leucine Zipper
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Immunohistochemistry (Paraffin-embedded sections) - c-Myc (phospho T358) antibody (ab30642)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab30642 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: 1/50 - 1/100.
ELISA
ELISA: 1/1000.ELISA: 1/1000.
IP: Use at an assay dependent dilution.
Target
Function
Participates in the regulation of gene transcription. Binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Seems to activate the transcription of growth-related genes.
Involvement in disease
Note=Overexpression of MYC is implicated in the etiology of a variety of hematopoietic tumors.
Note=A chromosomal aberration involving MYC may be a cause of a form of B-cell chronic lymphocytic leukemia. Translocation t(8;12)(q24;q22) with BTG1.
Defects in MYC are a cause of Burkitt lymphoma (BL) [MIM:113970]. A form of undifferentiated malignant lymphoma commonly manifested as a large osteolytic lesion in the jaw or as an abdominal mass. Note=Chromosomal aberrations involving MYC are usually found in Burkitt lymphoma. Translocations t(8;14), t(8;22) or t(2;8) which juxtapose MYC to one of the heavy or light chain immunoglobulin gene loci.
Sequence similarities
Contains 1 basic helix-loop-helix (bHLH) domain.
Post-translational
modifications
Phosphorylated by PRKDC. Phosphorylation at Thr-58 and Ser-62 by GSK3 is required for ubiquitination and degradation by the proteasome. Phosphorylation at Ser-329 by PIM2 leads to the stabilization of MYC (By similarity). Phosphorylation at Ser-62 by CDK2 prevents Ras-induced senescence.
Ubiquitinated by the SCF(FBXW7) complex when phosphorylated at Thr-58 and Ser-62, leading to its degradation by the proteasome. In the nucleoplasm, ubiquitination is counteracted by USP28, which interacts with isoform 1 of FBXW7 (FBW7alpha), leading to its deubiquitination and preventing degradation. In the nucleolus, however, ubiquitination is not counteracted by USP28, due to the lack of interaction between isoform 4 of FBXW7 (FBW7gamma) and USP28, explaining the selective MYC degradation in the nucleolus. Also polyubiquitinated by the DCX(TRUSS) complex.
Cellular localization
Nucleus > nucleoplasm. Nucleus > nucleolus.
Target information above from: UniProt accessionP01106
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Myc protein antibody
- bHLHe39 antibody
- c Myc antibody
see all
Database links
- Entrez Gene: 4609 Human
- Entrez Gene: 17869 Mouse
- Entrez Gene: 24577 Rat
- Omim: 190080 Human
- SwissProt: P01106 Human
- SwissProt: P01108 Mouse
- SwissProt: P09416 Rat
- Unigene: 202453 Human
see all
Anti-c-Myc (phospho T358) antibody images:
Immunohistochemistry (Paraffin-embedded sections) - c-Myc (phospho T358) antibody (ab30642)
Immunohistochemical analysis of paraffin-embedded breast carcinoma. Left: Using Phospho-Myc(Thr358) Antibody(ab30642); Right: The same antibody preincubated with synthesized phosphopeptide. Suggested dilution: 1:50 - 1:100
References for Anti-c-Myc (phospho T358) antibody (ab30642)
ab30642 has not yet been referenced specifically in any publications.
Publishing research using ab30642? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"