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Product name
Anti-c-Myc (phospho T58) antibody
See all c-Myc products (9) ...
Description
Rabbit polyclonal to c-Myc (phospho T58)
Tested applications
IHC-P, IP, ELISA, ICC/IF, WBmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Synthetic phospho-peptide derived from human Myc around the phosphorylation site of Threonine 58.
Epitope
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Positive control
Ovarian cancer cell lysate, breast carcinoma tissue.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Tumor biomarkers >> Oncoproteins
Cancer >> Oncoproteins/suppressors >> Oncoproteins >> Transcription factors
Cancer >> Cell cycle >> Cell differentiation
Epigenetics and Nuclear Signaling >> Transcription >> Transcription Factors
Stem Cells >> Signaling Pathways >> TGF beta >> Nuclear
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> HLH / Leucine Zipper
Applications
Show applications key
Our Abpromise guarantee covers the use of ab28842 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: 1/50 - 1/100.
IP: Use at an assay dependent dilution.
ELISA
ELISA: 1/1000.ELISA: 1/1000.
ICC/IF: Use a concentration of 1 µg/ml.
WB: 1/500 - 1/1000. Predicted molecular weight: 49 kDa.
Target
Function
Participates in the regulation of gene transcription. Binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Seems to activate the transcription of growth-related genes.
Involvement in disease
Note=Overexpression of MYC is implicated in the etiology of a variety of hematopoietic tumors.
Note=A chromosomal aberration involving MYC may be a cause of a form of B-cell chronic lymphocytic leukemia. Translocation t(8;12)(q24;q22) with BTG1.
Defects in MYC are a cause of Burkitt lymphoma (BL) [MIM:113970]. A form of undifferentiated malignant lymphoma commonly manifested as a large osteolytic lesion in the jaw or as an abdominal mass. Note=Chromosomal aberrations involving MYC are usually found in Burkitt lymphoma. Translocations t(8;14), t(8;22) or t(2;8) which juxtapose MYC to one of the heavy or light chain immunoglobulin gene loci.
Sequence similarities
Contains 1 basic helix-loop-helix (bHLH) domain.
Post-translational
modifications
Phosphorylated by PRKDC. Phosphorylation at Thr-58 and Ser-62 by GSK3 is required for ubiquitination and degradation by the proteasome. Phosphorylation at Ser-329 by PIM2 leads to the stabilization of MYC (By similarity). Phosphorylation at Ser-62 by CDK2 prevents Ras-induced senescence.
Ubiquitinated by the SCF(FBXW7) complex when phosphorylated at Thr-58 and Ser-62, leading to its degradation by the proteasome. In the nucleoplasm, ubiquitination is counteracted by USP28, which interacts with isoform 1 of FBXW7 (FBW7alpha), leading to its deubiquitination and preventing degradation. In the nucleolus, however, ubiquitination is not counteracted by USP28, due to the lack of interaction between isoform 4 of FBXW7 (FBW7gamma) and USP28, explaining the selective MYC degradation in the nucleolus. Also polyubiquitinated by the DCX(TRUSS) complex.
Cellular localization
Nucleus > nucleoplasm. Nucleus > nucleolus.
Target information above from: UniProt accessionP01106
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Myc protein antibody
- bHLHe39 antibody
- c Myc antibody
see all
Database links
- Entrez Gene: 4609 Human
- Entrez Gene: 17869 Mouse
- Omim: 190080 Human
- SwissProt: P01106 Human
- SwissProt: P01108 Mouse
- Unigene: 202453 Human
- Unigene: 2444 Mouse
Anti-c-Myc (phospho T58) antibody images:
Immunohistochemistry (Paraffin-embedded sections) - c-Myc (phospho T58) antibody (ab28842)
Immunohistochemical analysis of paraffin-embedded breast carcinoma. Left: Using Myc (Phospho-Thr58) Antibody; Right: The same antibody preincubated with synthesized phosphopeptide.
Western blot - c-Myc (phospho T58) antibody (ab28842)
Lane 1 : Anti-c-Myc (phospho T58) antibody (ab28842) at 1/500 dilution
Lane 2 : Anti-c-Myc (phospho T58) antibody (ab28842) at 1/500 dilution ( preincubated with synthesized non-
phosphopeptide
)
Lane 3 : Anti-c-Myc (phospho T58) antibody (ab28842) at 1/500 dilution ( preincubated with synthesized
phosphopeptide
)
Lane 1 : human ovarian cancer cell lysate
Lane 2 : human ovarian cancer cell lysate
Lane 3 : human ovarian cancer cell lysate
Predicted band size : 49 kDa
Observed band size : 51 kDa (why is the actual band size different from the predicted?)
Additional bands at : 120 kDa,55 kDa,86 kDa. We are unsure as to the identity of these extra bands.
Immunocytochemistry/ Immunofluorescence-c-Myc (phospho T58) antibody(ab28842)
ICC/IF image of ab28864 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28864, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-c-Myc (phospho T58) antibody (ab28842)
ab28842 has not yet been referenced specifically in any publications.
Publishing research using ab28842? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"