Overview

  • Product nameAnti-c Abl antibodySee all c Abl primary antibodies ...
  • Description
    Rabbit polyclonal to c Abl
  • SpecificityThis antibody detects endogenous levels of total c-Abl protein.
  • Tested applicationsSandwich ELISA, IHC-P, ELISA more details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    The antiserum was produced against synthesized non-phosphopeptide derived from human c-Abl around the phosphorylation site of tyrosine 412 (D-T-YP-T-A).

  • Positive control
    • Human breast carcinoma tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab47410 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
Sandwich ELISA Use at an assay dependent dilution. Can be paired for Sandwich ELISA with Mouse monoclonal [8E9] to c Abl (ab3227). Use as Detection antibody with recommended pair.
IHC-P Use at an assay dependent dilution.
ELISA 1/20000.

Target

  • FunctionProtein kinase that regulates key processes linked to cell growth and survival. Regulates cytoskeleton remodeling during cell differentiation, cell division and cell adhesion. Localizes to dynamic actin structures, and phosphorylates CRK and CRKL, DOK1, and other proteins controlling cytoskeleton dynamics. Regulates DNA repair potentially by activating the proapoptotic pathway when the DNA damage is too severe to be repaired. Phosphorylates PSMA7 that leads to an inhibition of proteasomal activity and cell cycle transition blocks.
  • Tissue specificityWidely expressed.
  • Involvement in diseaseNote=A chromosomal aberration involving ABL1 is a cause of chronic myeloid leukemia. Translocation t(9;22)(q34;q11) with BCR. The translocation produces a BCR-ABL found also in acute myeloid leukemia (AML) and acute lymphoblastic leukemia (ALL).
  • Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. ABL subfamily.
    Contains 1 protein kinase domain.
    Contains 1 SH2 domain.
    Contains 1 SH3 domain.
  • Post-translational
    modifications
    Phosphorylated by PRKDC (By similarity). DNA damage-induced activation of c-Abl requires the function of ATM and Ser-446 phosphorylation (By similarity). Phosphorylation on Thr-735 is required for binding 14-3-3 proteins for cytoplasmic translocation.
    Isoform IB is myristoylated on Gly-2.
  • Cellular localizationCytoplasm > cytoskeleton. Nucleus. Sequestered into the cytoplasm through interaction with 14-3-3 proteins and Nucleus membrane. The myristoylated c-ABL protein is reported to be nuclear.
  • Target information above from: UniProt accession P00519 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • Abelson Murine Leukemia Viral Oncogene Homolog 1 antibody
    • Abelson murine leukemia viral v abl oncogene homolog 1 antibody
    • Abelson tyrosine protein kinase 1 antibody
    • Abl 1 antibody
    • ABL antibody
    • Abl protein antibody
    • Abl1 antibody
    • ABL1_HUMAN antibody
    • bcr/abl antibody
    • Bcr/c abl oncogene protein antibody
    • c abl oncogene 1 non receptor tyrosine kinase antibody
    • c abl oncogene 1 receptor tyrosine kinase antibody
    • JTK 7 antibody
    • JTK7 antibody
    • p150 antibody
    • Proto oncogene tyrosine protein kinase ABL1 antibody
    • Proto-oncogene c-Abl antibody
    • Transformation gene oncogene ABL antibody
    • Tyrosine-protein kinase ABL1 antibody
    • v abl Abelson murine leukemia viral oncogene homolog 1 antibody
    • v abl antibody
    see all

Anti-c Abl antibody images

  • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue in the presence (right) and absence (left) of blocking peptide, using ab47410 at 1/50 dilution.

References for Anti-c Abl antibody (ab47410)

ab47410 has not yet been referenced specifically in any publications.

Product Wall

Application ELISA
Sample Human Cell (K562 Cell Lysate)
Specification K562 Cell Lysate
Blocking step BSA as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Type Sandwich (Detection)
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Submitted Dec 01 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"