Anti-c Abl (phospho Y412) antibody (ab194870)

Overview

  • Product name
    Anti-c Abl (phospho Y412) antibody
    See all c Abl primary antibodies
  • Description
    Rabbit polyclonal to c Abl (phospho Y412)
  • Tested applications
    Suitable for: ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human c Abl (phospho Y412). The exact sequence is proprietary.
    Database link: P00519

  • Positive control
    • Human breast carcinoma tissue and HeLa cells

Properties

Applications

Our Abpromise guarantee covers the use of ab194870 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/200.
IHC-P 1/50 - 1/100.

Target

  • Function
    Protein kinase that regulates key processes linked to cell growth and survival. Regulates cytoskeleton remodeling during cell differentiation, cell division and cell adhesion. Localizes to dynamic actin structures, and phosphorylates CRK and CRKL, DOK1, and other proteins controlling cytoskeleton dynamics. Regulates DNA repair potentially by activating the proapoptotic pathway when the DNA damage is too severe to be repaired. Phosphorylates PSMA7 that leads to an inhibition of proteasomal activity and cell cycle transition blocks.
  • Tissue specificity
    Widely expressed.
  • Involvement in disease
    Note=A chromosomal aberration involving ABL1 is a cause of chronic myeloid leukemia. Translocation t(9;22)(q34;q11) with BCR. The translocation produces a BCR-ABL found also in acute myeloid leukemia (AML) and acute lymphoblastic leukemia (ALL).
  • Sequence similarities
    Belongs to the protein kinase superfamily. Tyr protein kinase family. ABL subfamily.
    Contains 1 protein kinase domain.
    Contains 1 SH2 domain.
    Contains 1 SH3 domain.
  • Post-translational
    modifications
    Phosphorylated by PRKDC (By similarity). DNA damage-induced activation of c-Abl requires the function of ATM and Ser-446 phosphorylation (By similarity). Phosphorylation on Thr-735 is required for binding 14-3-3 proteins for cytoplasmic translocation.
    Isoform IB is myristoylated on Gly-2.
  • Cellular localization
    Cytoplasm > cytoskeleton. Nucleus. Sequestered into the cytoplasm through interaction with 14-3-3 proteins and Nucleus membrane. The myristoylated c-ABL protein is reported to be nuclear.
  • Information by UniProt
  • Database links
  • Alternative names
    • Abelson murine leukemia viral oncogene homolog 1 antibody
    • Abelson tyrosine protein kinase 1 antibody
    • Abl 1 antibody
    • ABL antibody
    • ABL proto oncogene 1 non receptor tyrosine kinase antibody
    • ABL1 antibody
    • ABL1_HUMAN antibody
    • bcr/abl antibody
    • bcr/c abl oncogene protein antibody
    • c ABL antibody
    • c abl oncogene 1 non receptor tyrosine kinase antibody
    • c abl oncogene 1 receptor tyrosine kinase antibody
    • c ABL1 antibody
    • JTK7 antibody
    • p150 antibody
    • Proto oncogene tyrosine protein kinase ABL1 antibody
    • Proto-oncogene c-Abl antibody
    • Tyrosine-protein kinase ABL1 antibody
    • v abl Abelson murine leukemia viral oncogene homolog 1 antibody
    • v abl antibody
    see all

Images

  • Immunofluorescent staining of methanol-fixed HeLa cells labeling c Abl (phospho Y412) using ab194870 at 1/100 dilution.

  • Immunohistochemical staining of Human breast carcinoma tissue labeling c Abl (phospho Y412) using ab194870 at 1/50 dilution.

References

ab194870 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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