Anti-c Abl (phospho Y412) antibody (ab47315)

Overview

  • Product nameAnti-c Abl (phospho Y412) antibody
    See all c Abl primary antibodies
  • Description
    Rabbit polyclonal to c Abl (phospho Y412)
  • Specificityab47315 detects endogenous levels of c-Abl only when phosphorylated at tyrosine 412.
  • Tested applicationsSuitable for: ICC/IF, WB, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    The antiserum was produced against synthesized phosphopeptide derived from human c-Abl around the phosphorylation site of tyrosine 412 (D-T-YP-T-A). Note: Two forms of c Abl are generated by alternative splicing. Tyr residue at position 412 is present in isoforms 1b of c Abl. The corresponding phosphorylation site in isoforms 1a is at position 393.

  • Positive control
    • Human breast carcinoma tissue

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab47315 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100.
WB Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
ELISA 1/4000.

Target

  • FunctionProtein kinase that regulates key processes linked to cell growth and survival. Regulates cytoskeleton remodeling during cell differentiation, cell division and cell adhesion. Localizes to dynamic actin structures, and phosphorylates CRK and CRKL, DOK1, and other proteins controlling cytoskeleton dynamics. Regulates DNA repair potentially by activating the proapoptotic pathway when the DNA damage is too severe to be repaired. Phosphorylates PSMA7 that leads to an inhibition of proteasomal activity and cell cycle transition blocks.
  • Tissue specificityWidely expressed.
  • Involvement in diseaseNote=A chromosomal aberration involving ABL1 is a cause of chronic myeloid leukemia. Translocation t(9;22)(q34;q11) with BCR. The translocation produces a BCR-ABL found also in acute myeloid leukemia (AML) and acute lymphoblastic leukemia (ALL).
  • Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. ABL subfamily.
    Contains 1 protein kinase domain.
    Contains 1 SH2 domain.
    Contains 1 SH3 domain.
  • Post-translational
    modifications
    Phosphorylated by PRKDC (By similarity). DNA damage-induced activation of c-Abl requires the function of ATM and Ser-446 phosphorylation (By similarity). Phosphorylation on Thr-735 is required for binding 14-3-3 proteins for cytoplasmic translocation.
    Isoform IB is myristoylated on Gly-2.
  • Cellular localizationCytoplasm > cytoskeleton. Nucleus. Sequestered into the cytoplasm through interaction with 14-3-3 proteins and Nucleus membrane. The myristoylated c-ABL protein is reported to be nuclear.
  • Information by UniProt
  • Database links
  • Alternative names
    • Abelson murine leukemia viral oncogene homolog 1 antibody
    • Abelson tyrosine protein kinase 1 antibody
    • Abl 1 antibody
    • ABL antibody
    • ABL proto oncogene 1 non receptor tyrosine kinase antibody
    • ABL1 antibody
    • ABL1_HUMAN antibody
    • bcr/abl antibody
    • bcr/c abl oncogene protein antibody
    • c ABL antibody
    • c abl oncogene 1 non receptor tyrosine kinase antibody
    • c abl oncogene 1 receptor tyrosine kinase antibody
    • c ABL1 antibody
    • JTK7 antibody
    • p150 antibody
    • Proto oncogene tyrosine protein kinase ABL1 antibody
    • Proto-oncogene c-Abl antibody
    • Tyrosine-protein kinase ABL1 antibody
    • v abl Abelson murine leukemia viral oncogene homolog 1 antibody
    • v abl antibody
    see all

Anti-c Abl (phospho Y412) antibody images

  • ab47315 staining human breast carcinoma by IHC-P (left hand panel). The right hand panel shows staining in the presence of phospho-peptide.


  • Performed under reducing conditions.

    Exposure time : 30 seconds

    See Abreview

  • ab47315 staining c Abl (phospho Y412) in human platelets by Immunocytochemistry/ Immunofluorescence. Cells were fixed with formaldehyde and permeabilized with 0.2% Triton x100 before blocking with 10% serum at 200C for 1 hour. Samples were incubated with primary antibody (1/100: in 10% goat serum) for 1 hour 20 minutes at 20°C. An Alexa Fluor®488-conjugated goat polyclonal to rabbit IgG used at dilution at 1/400 as secondary antibody.

    See Abreview

References for Anti-c Abl (phospho Y412) antibody (ab47315)

This product has been referenced in:
  • Xu J  et al. c-Abl mediates endothelial apoptosis induced by inhibition of integrins alphavbeta3 and alphavbeta5 and by disruption of actin. Blood 115:2709-18 (2010). WB ; Human . Read more (PubMed: 20124512) »
  • Jing Z  et al. Altered subcellular distribution of c-Abl in Alzheimer's disease. J Alzheimers Dis 17:409-22 (2009). WB, ICC/IF ; Human . Read more (PubMed: 19363261) »

See all 2 Publications for this product

Product Wall

Thank you for contacting us with your enquiry. Sometimes we areable to provide antibodies in custom buffers, however we do require that a bulk volume (10 or more vials) are purchased in one order. If you are interested in purchasing a bulk volume, I ca...

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Application Western blot
Sample Mouse Cell lysate - whole cell (platelet)
Loading amount 20 µg
Specification platelet
Treatment with or without MnCl2 20 minutes
Gel Running Conditions Reduced Denaturing (7.5 % polyacrylamide)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 22°C
Username

Dr. Ana Kasirer-Friede

Verified customer

Submitted Feb 16 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (platelet)
Specification platelet
Fixative Formaldehyde
Permeabilization Yes - 0.2% Triton X-100
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Jun 22 2009

The peptide pair ab5774 (one peptide is phosphorylated, the other is not), is not identical to the peptide used to generate antibody ab47315; it is a little bigger, approximately 10 amino acids according to our source. But it does contain the sequence ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"