Overview

  • Product nameAnti-c-Fos antibody
    See all c-Fos primary antibodies
  • Description
    Rabbit polyclonal to c-Fos
  • SpecificityRecognizes c-Fos and v-Fos.
  • Tested applicationsSuitable for: WB, ICC, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow, Cat, Dog, Pig
  • Immunogen

    Fusion protein:

    W LVQPALVSSV APSQTRAPHP FGVPAPSAGA YSRAGVVKTM TGGRAQSIGR RGKVEQLSPE EEEKRRIRRE RNKMAAAKCR

    , corresponding to N terminal amino acids 75-155 of Human c-Fos.

  • Positive control
    • HeLa nuclear extract.

Properties

Applications

Our Abpromise guarantee covers the use of ab18119 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ICC
IP
  • Application notes
    ICC: Use at a concentration of 5 - 10 µg/ml. This concentration has given positive results in HeLa cells that had been serum-starved and then serum-stimulated for 30 minutes prior to fixation with 4% paraformaldehyde followed by 1% Triton-X.
    IP: 4 - 10 µg immunoprecipitates c-Fos from 100µg of a RIPA lysate of human HeLa cells.
    WB: Use at a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 41 kDa.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionNuclear phosphoprotein which forms a tight but non-covalently linked complex with the JUN/AP-1 transcription factor. In the heterodimer, FOS and JUN/AP-1 basic regions each seems to interact with symmetrical DNA half sites. On TGF-beta activation, forms a multimeric SMAD3/SMAD4/JUN/FOS complex at the AP1/SMAD-binding site to regulate TGF-beta-mediated signaling. Has a critical function in regulating the Has a critical function in regulating the development of cells destined to form and maintain the skeleton. It is thought to have an important role in signal transduction, cell proliferation and differentiation.
    • Sequence similaritiesBelongs to the bZIP family. Fos subfamily.
      Contains 1 bZIP domain.
    • Post-translational
      modifications
      Phosphorylated in the C-terminal upon stimulation by nerve growth factor (NGF) and epidermal growth factor (EGF). Phosphorylated, in vitro, by MAPK and RSK1. Phosphorylation on both Ser-362 and Ser-374 by MAPK1/2 and RSK1/2 leads to protein stabilization with phosphorylation on Ser-374 being the major site for protein stabilization on NGF stimulation. Phosphorylation on Ser-362 and Ser-374 primes further phosphorylations on Thr-325 and Thr-331 through promoting docking of MAPK to the DEF domain. Phosphorylation on Thr-232, induced by HA-RAS, activates the transcriptional activity and antagonizes sumoylation. Phosphorylation on Ser-362 by RSK2 in osteoblasts contributes to osteoblast transformation.
      Constitutively sumoylated by SUMO1, SUMO2 and SUMO3. Desumoylated by SENP2. Sumoylation requires heterodimerization with JUN and is enhanced by mitogen stimulation. Sumoylation inhibits the AP-1 transcriptional activity and is, itself, inhibited by Ras-activated phosphorylation on Thr-232.
    • Cellular localizationNucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • Activator protein 1 antibody
      • AP 1 antibody
      • C FOS antibody
      • Cellular oncogene c fos antibody
      • Cellular oncogene fos antibody
      • FBJ murine osteosarcoma viral (v fos) oncogene homolog (oncogene FOS) antibody
      • FBJ murine osteosarcoma viral oncogene homolog antibody
      • FBJ murine osteosarcoma viral v fos oncogene homolog antibody
      • FBJ Osteosarcoma Virus antibody
      • FOS antibody
      • FOS protein antibody
      • FOS_HUMAN antibody
      • G0 G1 switch regulatory protein 7 antibody
      • G0/G1 switch regulatory protein 7 antibody
      • G0S7 antibody
      • Oncogene FOS antibody
      • p55 antibody
      • proto oncogene c Fos antibody
      • Proto oncogene protein c fos antibody
      • Proto-oncogene c-Fos antibody
      • v fos FBJ murine osteosarcoma viral oncogene homolog antibody
      see all

    References for Anti-c-Fos antibody (ab18119)

    ab18119 has not yet been referenced specifically in any publications.

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