|
|
|
|
Immunohistochemistry (Paraffin-embedded sections) - c-Fos antibody (ab53036)
(enlarge)
|
|
Western blot - c-Fos antibody (ab53036)
(enlarge)
|
|
|
|
|
Product Name
|
c-Fos antibody
|
| |
See all c-Fos antibodies (20)...
|
|
Product type
|
Primary antibodies
|
|
Description
|
Rabbit polyclonal to c-Fos
|
|
Immunogen
|
Synthetic peptide derived from human c-Fos
|
|
Reacts with
(species key)
|
Hu, Ms, Rat
|
|
Specificity
|
This antibody detects endogenous levels of total Fos protein
|
|
Tested applications
(see key)
|
ELISA, IHC-P, WB
|
|
|
Abreviews
|
|
|
|
Application notes
(see key)
|
Recommended dilutions
ELISA: 1/10000.
IHC-P: 1/50 - 1/100.
WB: 1/500 - 1/1000. Detects a band of approximately 50 kDa (predicted molecular weight: 41 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
|
|
Positive control
(see definition)
|
Human breast carcinoma tissue; HepG2 cell extract
|
|
Cellular localization
|
Nuclear
|
|
Research areas
|
Cancer >> Tumor biomarkers >> Oncoproteins
Cancer >> Oncoproteins/suppressors >> Oncoproteins >> Transcription factors
Chromatin and Nuclear Signaling >> Transcription >> Cancer susceptibility >> Proto-oncogenes
Chromatin and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> Leucine Zipper
Neuroscience >> Cell Type Marker >> Neuron marker >> Soma marker
|
|
Relevance
|
The Fos gene family consists of 4 members: Fos, FosB, FosL1, and FosL2. These genes encode leucine zipper proteins that can dimerize with proteins of the JUN family, thereby forming the transcription factor complex AP1. As such, the Fos proteins have been implicated as regulators of cell proliferation, differentiation, and transformation. In some cases, expression of the Fos gene has also been associated with apoptotic cell death. The human oncogene c-Fos is homologous to the Finkel-Biskis-Jinkins (FBJ) murine osteosarcoma virus oncogene. Fos was the first transcription factor identified that has a critical function in regulating the development of cells destined to form and maintain the skeleton.
Fos protein is routinely used as a marker of neuronal activation.
|
|
|
|
|
Database links
|
The links below go to external sites and will open in a new browser window |
|
Raised in
|
Rabbit
|
|
Clonality
|
Polyclonal
|
|
Isotype
|
IgG
|
|
Purity
|
Immunogen affinity purified
|
|
Storage buffer
|
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4 Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
|
|
Purification notes
|
Affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen
|
|
Form
|
Liquid
|
|
Concentration
|
1.000 mg/ml
|
|
Storage instructions
|
Store at -20°C. Stable for 12 months at -20°C
|
| |
At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this c-Fos antibody is on this datasheet. But please do contact us if you would like any reassurance! |
 |
|
|
See below for c-Fos antibody images, references, products related to ab53036 and other tools.
|
| |
c-Fos antibody images:
|
 Immunohistochemistry (Paraffin-embedded sections) - c-Fos antibody (ab53036)
Human breast carcinoma tissue labelled with ab53036 at 1/50 dilution; in the presence (+) and absence (-) of the immunizing peptide
|
| |
|
|
| |
| |
|
Search PubMed (MEDLINE) for references to c-Fos
|
| |
c-Fos antibody - more information
|
Customer reviews (feedback) regarding c-Fos antibody |
Customer FAQs regarding c-Fos antibody |
Protocols for c-Fos antibody |
Price and availability of products related to c-Fos antibody |
| |
c-Fos antibody - related products:
|
|
|
| |
c-Fos antibody - other tools:
|
Contact Abcam with a Technical Enquiry about c-Fos antibody |
| |
All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Pricing information loading
