Anti-c-Fos (phospho T232) antibody (ab17933)

Overview

  • Product nameAnti-c-Fos (phospho T232) antibody
    See all c-Fos primary antibodies
  • Description
    Rabbit polyclonal to c-Fos (phospho T232)
  • Tested applicationsICC, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide derived from the region of human c-Fos that contains Threonine 232. The sequence is conserved in mouse and rat.

  • Positive control
    • SKBR3 cells treated with EGF.

Properties

Applications

Our Abpromise guarantee covers the use of ab17933 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC
WB
  • Application notesICC: 1/300.
    WB: 1/1000. Detects a band of approximately 60 kDa (predicted molecular weight: 41 kDa). Another band is sometimes detected at approximately 100 kDa. This protein dimerizes with c-Jun, a ~40 kDa protein. This may account for the second band.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionNuclear phosphoprotein which forms a tight but non-covalently linked complex with the JUN/AP-1 transcription factor. In the heterodimer, FOS and JUN/AP-1 basic regions each seems to interact with symmetrical DNA half sites. On TGF-beta activation, forms a multimeric SMAD3/SMAD4/JUN/FOS complex at the AP1/SMAD-binding site to regulate TGF-beta-mediated signaling. Has a critical function in regulating the Has a critical function in regulating the development of cells destined to form and maintain the skeleton. It is thought to have an important role in signal transduction, cell proliferation and differentiation.
    • Sequence similaritiesBelongs to the bZIP family. Fos subfamily.
      Contains 1 bZIP domain.
    • Post-translational
      modifications
      Phosphorylated in the C-terminal upon stimulation by nerve growth factor (NGF) and epidermal growth factor (EGF). Phosphorylated, in vitro, by MAPK and RSK1. Phosphorylation on both Ser-362 and Ser-374 by MAPK1/2 and RSK1/2 leads to protein stabilization with phosphorylation on Ser-374 being the major site for protein stabilization on NGF stimulation. Phosphorylation on Ser-362 and Ser-374 primes further phosphorylations on Thr-325 and Thr-331 through promoting docking of MAPK to the DEF domain. Phosphorylation on Thr-232, induced by HA-RAS, activates the transcriptional activity and antagonizes sumoylation. Phosphorylation on Ser-362 by RSK2 in osteoblasts contributes to osteoblast transformation.
      Constitutively sumoylated by SUMO1, SUMO2 and SUMO3. Desumoylated by SENP2. Sumoylation requires heterodimerization with JUN and is enhanced by mitogen stimulation. Sumoylation inhibits the AP-1 transcriptional activity and is, itself, inhibited by Ras-activated phosphorylation on Thr-232.
    • Cellular localizationNucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • Activator protein 1 antibody
      • AP 1 antibody
      • C FOS antibody
      • Cellular oncogene c fos antibody
      • Cellular oncogene fos antibody
      • Cellular oncogene fos antibody
      • FBJ murine osteosarcoma viral (v fos) oncogene homolog (oncogene FOS) antibody
      • FBJ murine osteosarcoma viral oncogene homolog antibody
      • FBJ murine osteosarcoma viral v fos oncogene homolog antibody
      • FBJ murine osteosarcoma viral v fos oncogene homolog antibody
      • FBJ Osteosarcoma Virus antibody
      • FBJ Osteosarcoma Virus antibody
      • FOS antibody
      • FOS protein antibody
      • FOS_HUMAN antibody
      • G0 G1 switch regulatory protein 7 antibody
      • G0 G1 switch regulatory protein 7 antibody
      • G0/G1 switch regulatory protein 7 antibody
      • G0S7 antibody
      • G0S7 antibody
      • Oncogene FOS antibody
      • Oncogene FOS antibody
      • p55 antibody
      • proto oncogene c Fos antibody
      • Proto oncogene protein c fos antibody
      • Proto oncogene protein c fos antibody
      • Proto-oncogene c-Fos antibody
      • v fos FBJ murine osteosarcoma viral oncogene homolog antibody
      • v fos FBJ murine osteosarcoma viral oncogene homolog antibody
      see all

    Anti-c-Fos (phospho T232) antibody images



    • Predicted band size : 41 kDa


      Lysates prepared from SKBR3 cells were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 3% Milk-TBST buffer for one hour at room temperature, and incubated with c-Fos [pT232] antibody for two hours at room temperature in a 3% Milk-TBST buffer, following prior incubation with: no peptide (1), the non-phosphopeptide corresponding to the immunogen (2), a generic phosphothreonine-containing peptide (3), or, the phosphopeptide immunogen (4). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG HRP conjugate and bands were detected using the Pierce SuperSignal™ method. The data show that only the peptide corresponding to c-Fos [pT232] blocks the signal, thereby demonstrating the specificity of the antibody. While c-Fos [pT232] is phosphorylated in the basal state of SKBR3, EGF treatment induced an increase in the phosphorylation signal (data not shown).
    • ab17933 detecting nuclear staining of phosphorylated T232 c-Fos (green colour) in [A] unstimulated cells and [B] NGF stimulated (100 ng/ml for 2 min) adult dorsal root ganglion (DRG) cells cultured for 2 days. ab17933 was used at 1/300 (incubated for 2h at room temperature). Secondary antibody used is anti-rabbit alexa fluor 488 (at 1/1000 for 1hr at room temperature). The cytoplasm of these DRG cells are immunofluorescent for the neuronal marker beta III tubulin (orange colour). The cell diameter is approx. 25µm.

    References for Anti-c-Fos (phospho T232) antibody (ab17933)

    This product has been referenced in:
    • Lutay N  et al. Bacterial control of host gene expression through RNA polymerase II. J Clin Invest 123:2366-79 (2013). WB ; Human . Read more (PubMed: 23728172) »
    • Shields SD  et al. Sodium channel Na(v)1.7 is essential for lowering heat pain threshold after burn injury. J Neurosci 32:10819-32 (2012). Read more (PubMed: 22875917) »

    See all 6 Publications for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (huh-7)
    Loading amount 20 µg
    Specification huh-7
    Treatment cells expressing viral protein on pcDNA 3.1 vector
    Gel Running Conditions Reduced Denaturing
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Username

    Abcam user community

    Verified customer

    Submitted Jan 15 2010

    Thank you for your reply. Please see the response from the originator of the antibody. "We are very confident of the specificity of this antibody which had has been thoroughly tested by us (including on c-fos IP sample using Pan antibodies from o...

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    Thank you for your reply. The suggested buffer is a modified RIPA buffer. I would agree that you should theoretically not be seeing dimers, but the originator mentioned that they had seen the same problem with other researchers who were using a buf...

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    Thank you for your enquiry. c-Fos dimerizes with c-Jun (~40kDa), so this is what the band around 100 kDa may be. One consideration is why no band was observed at 60 kDa. I have obtained the Cell Lysis buffer recipe from the originator of the ant...

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    Thank you for your enquiry. Ab27793 has not yet been tested for application in IF (only Western blotting so far)but ab17933 - Rabbit polyclonal to c-Fos (phospho T232) - has been tested in IF. There is an image on the online datasheet with more more in...

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    Thank you for your enquiry and patience. I contacted our source for this antibody and ab17933 detects a band at approximately 60 kDa. c-Fos is an approximately 60 kDa immediate early gene product. Regarding the Western blot image with the peptide c...

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