Anti-c-Fos (phospho T232) antibody (ab43175)
Key features and details
- Rabbit polyclonal to c-Fos (phospho T232)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-c-Fos (phospho T232) antibody
See all c-Fos primary antibodies -
Description
Rabbit polyclonal to c-Fos (phospho T232) -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Sheep, Pig, Chimpanzee -
Immunogen
Synthetic peptide corresponding to Human c-Fos aa 200-300 (phospho T232) conjugated to keyhole limpet haemocyanin.
(Peptide available asab43652) -
Positive control
- This antibody gave a positive signal in the following human whole cell lysates: A431 - EGF treated, HeLa, Hela - Bleomycin Treated (40U/ml), Hela - Hydroxyurea Treated (48hr, 2uM). This antibody also gave a positive signal against the Full length Recombinant FOS protein (Tagged).
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Assay kits
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab43175 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 95 kDa (predicted molecular weight: 41 kDa).
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Notes |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 95 kDa (predicted molecular weight: 41 kDa). |
Target
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Function
Nuclear phosphoprotein which forms a tight but non-covalently linked complex with the JUN/AP-1 transcription factor. In the heterodimer, FOS and JUN/AP-1 basic regions each seems to interact with symmetrical DNA half sites. On TGF-beta activation, forms a multimeric SMAD3/SMAD4/JUN/FOS complex at the AP1/SMAD-binding site to regulate TGF-beta-mediated signaling. Has a critical function in regulating the development of cells destined to form and maintain the skeleton. It is thought to have an important role in signal transduction, cell proliferation and differentiation. -
Sequence similarities
Belongs to the bZIP family. Fos subfamily.
Contains 1 bZIP domain. -
Post-translational
modificationsPhosphorylated in the C-terminal upon stimulation by nerve growth factor (NGF) and epidermal growth factor (EGF). Phosphorylated, in vitro, by MAPK and RSK1. Phosphorylation on both Ser-362 and Ser-374 by MAPK1/2 and RSK1/2 leads to protein stabilization with phosphorylation on Ser-374 being the major site for protein stabilization on NGF stimulation. Phosphorylation on Ser-362 and Ser-374 primes further phosphorylations on Thr-325 and Thr-331 through promoting docking of MAPK to the DEF domain. Phosphorylation on Thr-232, induced by HA-RAS, activates the transcriptional activity and antagonizes sumoylation. Phosphorylation on Ser-362 by RSK2 in osteoblasts contributes to osteoblast transformation.
Constitutively sumoylated by SUMO1, SUMO2 and SUMO3. Desumoylated by SENP2. Sumoylation requires heterodimerization with JUN and is enhanced by mitogen stimulation. Sumoylation inhibits the AP-1 transcriptional activity and is, itself, inhibited by Ras-activated phosphorylation on Thr-232. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 2353 Human
- Entrez Gene: 100144486 Pig
- Omim: 164810 Human
- SwissProt: P01100 Human
- SwissProt: O97930 Pig
- SwissProt: O02761 Sheep
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Alternative names
- Activator protein 1 antibody
- AP 1 antibody
- C FOS antibody
see all
Images
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All lanes : Anti-c-Fos (phospho T232) antibody (ab43175) at 1 µg/ml
Lane 1 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate - EGF treated
Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 3 : Hela Whole Cell Lysate - Bleomycin Treated (40U/ml)
Lane 4 : Hela Whole Cell Lysate - Hydroxyurea Treated (48hr, 2uM)
Lane 5 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate - EGF treated withHuman c-Fos (phospho T232) peptide (ab43652) at 1 µg/ml
Lane 6 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate withHuman c-Fos (phospho T232) peptide (ab43652) at 1 µg/ml
Lane 7 : Hela Whole Cell Lysate - Bleomycin Treated (40U/ml)
withHuman c-Fos (phospho T232) peptide (ab43652) at 1 µg/ml
Lane 8 : Hela Whole Cell Lysate - Hydroxyurea Treated (48hr, 2uM) withHuman c-Fos (phospho T232) peptide (ab43652) at 1 µg/ml
Lane 9 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate - EGF treated with Human c-Fos peptide (ab60297) at 1 µg/ml
Lane 10 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate with Human c-Fos peptide (ab60297) at 1 µg/ml
Lane 11 : Hela Whole Cell Lysate - Bleomycin Treated (40U/ml)
with Human c-Fos peptide (ab60297) at 1 µg/ml
Lane 12 : Hela Whole Cell Lysate - Hydroxyurea Treated (48hr, 2uM) with Human c-Fos peptide (ab60297) at 1 µg/ml
Lysates/proteins at 10 µg per lane.
Secondary
Lanes 1 & 12 : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Lanes 2-11 : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 41 kDa
ab43175 detects a 95 kDa band in western blot. This is consistent with results obtained by other commercially available antibodies to this target, as c-Fos has been reported to dimerise with the c-Jun protein hence migration at a higher molecular weight than predicted. -
Anti-c-Fos (phospho T232) antibody (ab43175) at 1 µg/ml +
Recombinant Human c-Fos protein (ab56280) at 0.1 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 41 kDa
Observed band size: 78 kDa why is the actual band size different from the predicted?
ab56280, c-Fos protein (Tagged), has a predicted molecular weight of 78 kDa in SDS-PAGE.
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab43175 has been referenced in 2 publications.
- Araújo FC et al. Expression of the proto-oncogene c-fos and the immunolocalization of c-fos, phosphorylated c-fos and estrogen receptor beta in the human testis. Histol Histopathol 24:1515-22 (2009). IHC-P ; Human . PubMed: 19795350
- Morsch DM et al. c-fos gene and protein expression in pelvic endometriosis: a local marker of estrogen action. J Mol Histol 40:53-8 (2009). IHC-P ; Human . PubMed: 19199093