• Product nameAnti-c-IAP1 and c-IAP2 antibody
  • Description
    Rabbit polyclonal to c-IAP1 and c-IAP2
  • SpecificityThis antibody detects both c-IAP1 and c-IAP2.
  • Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to 14 C terminal amino acids of Human cIAP1

  • Positive control
    • Human lung cell lysate can be used as positive control.


Associated products


Our Abpromise guarantee covers the use of ab25939 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 70 kDa.
IHC-P Use a concentration of 10 µg/ml.

Anti-c-IAP1 and c-IAP2 antibody images

  • Lane 1 : Anti-c-IAP1 and c-IAP2 antibody (ab25939) at 1 µg/ml
    Lane 2 : Anti-c-IAP1 and c-IAP2 antibody (ab25939) at 2 µg/ml
    Lane 3 : Anti-c-IAP1 and c-IAP2 antibody (ab25939) at 4 µg/ml

    Lane 1 : human lung lysate
    Lane 2 : human lung lysate
    Lane 3 : human lung lysate
  • Lane 1 : Anti-c-IAP1 and c-IAP2 antibody (ab25939) at 1 µg/ml
    Lane 2 : Anti-c-IAP1 and c-IAP2 antibody (ab25939) at 2 µg/ml
    Lane 3 : Anti-c-IAP1 and c-IAP2 antibody (ab25939) at 4 µg/ml

    Lane 1 : Human lung lysate
    Lane 2 : Human lung lysate
    Lane 3 : Human lung lysate
  • ab25939 at 10µg/ml staining cIAP1 in human lung cells by IHC 

  • ICC/IF image of ab25939 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab25939, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Immunofluorescence of cIAP in Human Lung cells using ab25939 at 20 ug/ml.

References for Anti-c-IAP1 and c-IAP2 antibody (ab25939)

This product has been referenced in:
  • Gualco E  et al. IGF-IR-dependent expression of Survivin is required for T-antigen-mediated protection from apoptosis and proliferation of neural progenitors. Cell Death Differ 17:439-51 (2010). IHC-P ; Mouse . Read more (PubMed: 19834489) »
  • Zhao TT  et al. hnRNP A1 regulates UV-induced NF-kappaB signalling through destabilization of cIAP1 mRNA. Cell Death Differ : (2008). WB ; Human . Read more (PubMed: 18846111) »

See all 2 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HEK293)
Loading amount 25 µg
Specification HEK293
Gel Running Conditions Non-reduced Denaturing (10% gel)
Blocking step Milk as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 21°C

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Submitted Jul 23 2012