Overview

  • Product name
    Anti-c-IAP1 and c-IAP2 antibody
  • Description
    Rabbit polyclonal to c-IAP1 and c-IAP2
  • Host species
    Rabbit
  • Specificity
    This antibody detects both c-IAP1 and c-IAP2.
  • Tested applications
    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to 14 C terminal amino acids of Human cIAP1

  • Positive control
    • Human lung cell lysate can be used as positive control.

Properties

Applications

Our Abpromise guarantee covers the use of ab25939 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 70 kDa.
IHC-P Use a concentration of 10 µg/ml.

Images

  • Lane 1 : Anti-c-IAP1 and c-IAP2 antibody (ab25939) at 1 µg/ml
    Lane 2 : Anti-c-IAP1 and c-IAP2 antibody (ab25939) at 2 µg/ml
    Lane 3 : Anti-c-IAP1 and c-IAP2 antibody (ab25939) at 4 µg/ml

    All lanes : human lung lysate
  • Lane 1 : Anti-c-IAP1 and c-IAP2 antibody (ab25939) at 1 µg/ml
    Lane 2 : Anti-c-IAP1 and c-IAP2 antibody (ab25939) at 2 µg/ml
    Lane 3 : Anti-c-IAP1 and c-IAP2 antibody (ab25939) at 4 µg/ml

    All lanes : Human lung lysate
  • ab25939 at 10µg/ml staining cIAP1 in human lung cells by IHC 

  • ICC/IF image of ab25939 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab25939, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Immunofluorescence of cIAP in Human Lung cells using ab25939 at 20 ug/ml.

References

This product has been referenced in:
  • Yan H  et al. NR4A1-induced increase in the sensitivity of a human gastric cancer line to TNFa-mediated apoptosis is associated with the inhibition of JNK/Parkin-dependent mitophagy. Int J Oncol 52:367-378 (2018). Read more (PubMed: 29207128) »
  • Woo M  et al. Kimchi attenuates fatty streak formation in the aorta of low-density lipoprotein receptor knockout mice via inhibition of endoplasmic reticulum stress and apoptosis. Nutr Res Pract 11:445-451 (2017). Read more (PubMed: 29209454) »

See all 5 Publications for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293)
Loading amount
25 µg
Specification
HEK293
Gel Running Conditions
Non-reduced Denaturing (10% gel)
Blocking step
Milk as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 21°C
Username

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Verified customer

Submitted Jul 23 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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