Overview

  • Product nameAnti-c-Jun antibody [5B1]
    See all c-Jun primary antibodies
  • Description
    Mouse monoclonal [5B1] to c-Jun
  • Tested applicationsSuitable for: WB, ELISA, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human, Monkey
  • Immunogen

    Recombinant fragment of Human c-Jun expressed in E. Coli.

  • Positive control
    • Recombinant c-Jun protein, NIH3T3 and COS7 cell lysates, Human intima cancer and Human rectum cancer tissues, PC2 cells. HepG2 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab119944 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Detects a band of approximately 43 kDa (predicted molecular weight: 35 kDa).
ELISA 1/10000.
IHC-P 1/200 - 1/1000.
ICC/IF 1/200 - 1/1000.

Target

  • FunctionTranscription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. Promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. Involved in activated KRAS-mediated transcriptional activation of USP28 in colorectal cancer (CRC) cells (PubMed:24623306). Binds to the USP28 promoter in colorectal cancer (CRC) cells (PubMed:24623306).
  • Sequence similaritiesBelongs to the bZIP family. Jun subfamily.
    Contains 1 bZIP (basic-leucine zipper) domain.
  • Post-translational
    modifications
    Ubiquitinated by the SCF(FBXW7), leading to its degradation. Ubiquitination takes place following phosphorylation, that promotes interaction with FBXW7.
    Phosphorylated by CaMK4 and PRKDC; phosphorylation enhances the transcriptional activity. Phosphorylated by HIPK3. Phosphorylated by DYRK2 at Ser-243; this primes the protein for subsequent phosphorylation by GSK3B at Thr-239. Phosphorylated at Thr-239, Ser-243 and Ser-249 by GSK3B; phosphorylation reduces its ability to bind DNA. Phosphorylated by PAK2 at Thr-2, Thr-8, Thr-89, Thr-93 and Thr-286 thereby promoting JUN-mediated cell proliferation and transformation. Phosphorylated by PLK3 following hypoxia or UV irradiation, leading to increase DNA-binding activity.
    Acetylated at Lys-271 by EP300.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Activator protein 1 antibody
    • AP 1 antibody
    • AP1 antibody
    • cJun antibody
    • Enhancer Binding Protein AP1 antibody
    • Jun Activation Domain Binding Protein antibody
    • JUN antibody
    • Jun oncogene antibody
    • JUN protein antibody
    • Jun proto oncogene antibody
    • JUN_HUMAN antibody
    • JUNC antibody
    • Oncogene JUN antibody
    • p39 antibody
    • Proto oncogene c jun antibody
    • Proto oncogene cJun antibody
    • Proto-oncogene c-jun antibody
    • Transcription Factor AP 1 antibody
    • Transcription factor AP-1 antibody
    • Transcription Factor AP1 antibody
    • V jun avian sarcoma virus 17 oncogene homolog antibody
    • V jun sarcoma virus 17 oncogene homolog (avian) antibody
    • V jun sarcoma virus 17 oncogene homolog antibody
    • V-jun avian sarcoma virus 17 oncogene homolog antibody
    • vJun Avian Sarcoma Virus 17 Oncogene Homolog antibody
    see all

Anti-c-Jun antibody [5B1] images

  • Anti-c-Jun antibody [5B1] (ab119944) at 1/500 dilution + Recombinant c-Jun protein

    Predicted band size : 35 kDa
    Observed band size : 43 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-c-Jun antibody [5B1] (ab119944) at 1/500 dilution

    Lane 1 : NIH3T3 cell lysate
    Lane 2 : COS7 cell lysate


    Predicted band size : 35 kDa
    Observed band size : 43 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemical analysis of paraffin-embedded Human intima canncer tissues using ab119944 at a dilution of 1/200 with DAB staining.
  • Immunohistochemical analysis of paraffin-embedded Human rectum canncer tissues using ab119944 at a dilution of 1/200 with DAB staining.
  • Immunofluorescence analysis of PC-2 cells using ab119944 at a dilution of 1/200 (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
  • ELISA, using ab119944 at a dilution of 1/10000.

References for Anti-c-Jun antibody [5B1] (ab119944)

This product has been referenced in:

See 1 Publication for this product

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