Overview

  • Product name
    Anti-c-Jun antibody [5B1]
    See all c-Jun primary antibodies
  • Description
    Mouse monoclonal [5B1] to c-Jun
  • Tested applications
    Suitable for: WB, ELISA, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human, Monkey
  • Immunogen

    Recombinant fragment of Human c-Jun expressed in E. Coli.

  • Positive control
    • Recombinant c-Jun protein, NIH3T3 and COS7 cell lysates, Human intima cancer and Human rectum cancer tissues, PC2 cells. HepG2 cells.
  • General notes

    This product was changed from ascites to supernatant. Lot no’s high than GR229034-11 are from Tissue Culture Supernatant

Properties

Applications

Our Abpromise guarantee covers the use of ab119944 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Detects a band of approximately 43 kDa (predicted molecular weight: 35 kDa).
ELISA 1/10000.
IHC-P 1/200 - 1/1000.
ICC/IF 1/200 - 1/1000.

Target

  • Function
    Transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. Promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. Involved in activated KRAS-mediated transcriptional activation of USP28 in colorectal cancer (CRC) cells (PubMed:24623306). Binds to the USP28 promoter in colorectal cancer (CRC) cells (PubMed:24623306).
  • Sequence similarities
    Belongs to the bZIP family. Jun subfamily.
    Contains 1 bZIP (basic-leucine zipper) domain.
  • Post-translational
    modifications
    Ubiquitinated by the SCF(FBXW7), leading to its degradation. Ubiquitination takes place following phosphorylation, that promotes interaction with FBXW7.
    Phosphorylated by CaMK4 and PRKDC; phosphorylation enhances the transcriptional activity. Phosphorylated by HIPK3. Phosphorylated by DYRK2 at Ser-243; this primes the protein for subsequent phosphorylation by GSK3B at Thr-239. Phosphorylated at Thr-239, Ser-243 and Ser-249 by GSK3B; phosphorylation reduces its ability to bind DNA. Phosphorylated by PAK2 at Thr-2, Thr-8, Thr-89, Thr-93 and Thr-286 thereby promoting JUN-mediated cell proliferation and transformation. Phosphorylated by PLK3 following hypoxia or UV irradiation, leading to increase DNA-binding activity.
    Acetylated at Lys-271 by EP300.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Activator protein 1 antibody
    • AP 1 antibody
    • AP1 antibody
    • cJun antibody
    • Enhancer Binding Protein AP1 antibody
    • Jun Activation Domain Binding Protein antibody
    • JUN antibody
    • Jun oncogene antibody
    • JUN protein antibody
    • Jun proto oncogene antibody
    • JUN_HUMAN antibody
    • JUNC antibody
    • Oncogene JUN antibody
    • p39 antibody
    • Proto oncogene c jun antibody
    • Proto oncogene cJun antibody
    • Proto-oncogene c-jun antibody
    • Transcription Factor AP 1 antibody
    • Transcription factor AP-1 antibody
    • Transcription Factor AP1 antibody
    • V jun avian sarcoma virus 17 oncogene homolog antibody
    • V jun sarcoma virus 17 oncogene homolog (avian) antibody
    • V jun sarcoma virus 17 oncogene homolog antibody
    • V-jun avian sarcoma virus 17 oncogene homolog antibody
    • vJun Avian Sarcoma Virus 17 Oncogene Homolog antibody
    see all

Images

  • Anti-c-Jun antibody [5B1] (ab119944) at 1/500 dilution + Recombinant c-Jun protein

    Predicted band size : 35 kDa
    Observed band size : 43 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-c-Jun antibody [5B1] (ab119944) at 1/500 dilution

    Lane 1 : NIH3T3 cell lysate
    Lane 2 : COS7 cell lysate


    Predicted band size : 35 kDa
    Observed band size : 43 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemical analysis of paraffin-embedded Human intima canncer tissues using ab119944 at a dilution of 1/200 with DAB staining.
  • Immunohistochemical analysis of paraffin-embedded Human rectum canncer tissues using ab119944 at a dilution of 1/200 with DAB staining.
  • Immunofluorescence analysis of PC-2 cells using ab119944 at a dilution of 1/200 (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
  • ELISA, using ab119944 at a dilution of 1/10000.

References

This product has been referenced in:

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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