The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration. PubMed: 22615549
1/500 - 1/1000. Detects a band of approximately 42 kDa (predicted molecular weight: 36 kDa).
Use at an assay dependent concentration.
FunctionTranscription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. Promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. Involved in activated KRAS-mediated transcriptional activation of USP28 in colorectal cancer (CRC) cells (PubMed:24623306). Binds to the USP28 promoter in colorectal cancer (CRC) cells (PubMed:24623306).
Sequence similaritiesBelongs to the bZIP family. Jun subfamily. Contains 1 bZIP (basic-leucine zipper) domain.
Post-translational modificationsUbiquitinated by the SCF(FBXW7), leading to its degradation. Ubiquitination takes place following phosphorylation, that promotes interaction with FBXW7. Phosphorylated by CaMK4 and PRKDC; phosphorylation enhances the transcriptional activity. Phosphorylated by HIPK3. Phosphorylated by DYRK2 at Ser-243; this primes the protein for subsequent phosphorylation by GSK3B at Thr-239. Phosphorylated at Thr-239, Ser-243 and Ser-249 by GSK3B; phosphorylation reduces its ability to bind DNA. Phosphorylated by PAK2 at Thr-2, Thr-8, Thr-89, Thr-93 and Thr-286 thereby promoting JUN-mediated cell proliferation and transformation. Phosphorylated by PLK3 following hypoxia or UV irradiation, leading to increase DNA-binding activity. Acetylated at Lys-271 by EP300.
ab31367 at 1:100, Immunohistochemical analysis of paraffin-embedded breast carcinoma. Left: c-Jun antibody, Right: Preincubated with synthesized peptide.
Western blot - c-Jun antibody (ab31367)
All lanes : Anti-c-Jun antibody (ab31367) at 1/1000 dilution
Lane 1 : HeLa cells lysate, preincubated with immunogen (synthetic non-phosphopeptide) Lane 2 : HeLa cells lysate
Predicted band size : 36 kDa
References for Anti-c-Jun antibody (ab31367)
This product has been referenced in:
Winter C et al. Google goes cancer: improving outcome prediction for cancer patients by network-based ranking of marker genes. PLoS Comput Biol8:e1002511 (2012).
Read more (PubMed: 22615549) »
Samson WK et al. Neuronostatin encoded by the somatostatin gene regulates neuronal, cardiovascular, and metabolic functions. J Biol Chem283:31949-59 (2008).
Read more (PubMed: 18753129) »