Anti-c-Jun (phospho S63) antibody [Y172] (ab32385)


  • Product nameAnti-c-Jun (phospho S63) antibody [Y172]
    See all c-Jun primary antibodies
  • Description
    Rabbit monoclonal [Y172] to c-Jun (phospho S63)
  • SpecificityThe antibody only detects c-Jun phosphorylated on Serine 63.
  • Tested applicationsSuitable for: WB, IHC-P, ICCmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow
  • Immunogen

    A synthetic phospho peptide corresponding to residues surrounding Ser63 of human c-Jun.

  • Positive control
    • WB: NIH 3T3 cell lysate. IHC-P: Human breast carcinoma.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.


    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    A trial size is available to purchase for this antibody.



Our Abpromise guarantee covers the use of ab32385 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 42 kDa (predicted molecular weight: 36 kDa).
IHC-P 1/50 - 1/100.
ICC 1/100 - 1/200.
  • Application notesIs unsuitable for Flow Cyt or IP.
  • Target

    • FunctionTranscription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. Promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. Involved in activated KRAS-mediated transcriptional activation of USP28 in colorectal cancer (CRC) cells (PubMed:24623306). Binds to the USP28 promoter in colorectal cancer (CRC) cells (PubMed:24623306).
    • Sequence similaritiesBelongs to the bZIP family. Jun subfamily.
      Contains 1 bZIP (basic-leucine zipper) domain.
    • Post-translational
      Ubiquitinated by the SCF(FBXW7), leading to its degradation. Ubiquitination takes place following phosphorylation, that promotes interaction with FBXW7.
      Phosphorylated by CaMK4 and PRKDC; phosphorylation enhances the transcriptional activity. Phosphorylated by HIPK3. Phosphorylated by DYRK2 at Ser-243; this primes the protein for subsequent phosphorylation by GSK3B at Thr-239. Phosphorylated at Thr-239, Ser-243 and Ser-249 by GSK3B; phosphorylation reduces its ability to bind DNA. Phosphorylated by PAK2 at Thr-2, Thr-8, Thr-89, Thr-93 and Thr-286 thereby promoting JUN-mediated cell proliferation and transformation. Phosphorylated by PLK3 following hypoxia or UV irradiation, leading to increase DNA-binding activity.
      Acetylated at Lys-271 by EP300.
    • Cellular localizationNucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • Activator protein 1 antibody
      • AP 1 antibody
      • AP1 antibody
      • cJun antibody
      • Enhancer Binding Protein AP1 antibody
      • Jun Activation Domain Binding Protein antibody
      • JUN antibody
      • Jun oncogene antibody
      • JUN protein antibody
      • Jun proto oncogene antibody
      • JUN_HUMAN antibody
      • JUNC antibody
      • Oncogene JUN antibody
      • p39 antibody
      • Proto oncogene c jun antibody
      • Proto oncogene cJun antibody
      • Proto-oncogene c-jun antibody
      • Transcription Factor AP 1 antibody
      • Transcription factor AP-1 antibody
      • Transcription Factor AP1 antibody
      • V jun avian sarcoma virus 17 oncogene homolog antibody
      • V jun sarcoma virus 17 oncogene homolog (avian) antibody
      • V jun sarcoma virus 17 oncogene homolog antibody
      • V-jun avian sarcoma virus 17 oncogene homolog antibody
      • vJun Avian Sarcoma Virus 17 Oncogene Homolog antibody
      see all

    Anti-c-Jun (phospho S63) antibody [Y172] images

    • All lanes : Anti-c-Jun (phospho S63) antibody [Y172] (ab32385) at 1/10000 dilution

      Lane 1 : NIH 3T3 cell lysate not treated with ultraviolet
      Lane 2 : NIH 3T3 cell lysate treated with ultraviolet
      Lane 3 : NIH 3T3 cell lysate not treated with Anisomycin
      Lane 4 : NIH 3T3 cell lysate treated with Anisomycin

      Predicted band size : 36 kDa
      Observed band size : 42 kDa (why is the actual band size different from the predicted?)
      NIH3T3 cells were treated with 25 ug/ml Anisomycin for 15 minutes at 37°C.
    • Ab32385, at a 1/50 dilution, staining c-Jun in paraffin embedded human breast carcinoma tissue by Immunohistochemistry.

    References for Anti-c-Jun (phospho S63) antibody [Y172] (ab32385)

    This product has been referenced in:
    • Suwei D  et al. NLK functions to maintain proliferation and stemness of NSCLC and is a target of metformin. J Hematol Oncol 8:120 (2015). Human . Read more (PubMed: 26503334) »
    • Katanov C  et al. Regulation of the inflammatory profile of stromal cells in human breast cancer: prominent roles for TNF-a and the NF-?B pathway. Stem Cell Res Ther 6:87 (2015). WB ; Human . Read more (PubMed: 25928089) »

    See all 11 Publications for this product

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