• Product nameAnti-c-Jun (phospho T91) antibody
    See all c-Jun primary antibodies
  • Description
    Rabbit polyclonal to c-Jun (phospho T91)
  • Specificityc-Jun (Phospho-Thr91) Antibody detects endogenous levels of c-Jun only when phosphorylated at Thr91.
  • Tested applicationsSuitable for: IP, WB, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human c-Jun (phospho T91). The immunogen range is within aa 58-107.
    Database link: P05412



Our Abpromise guarantee covers the use of ab28853 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.
WB 1/500 - 1/1000. Predicted molecular weight: 36 kDa.
IHC-P 1/50 - 1/100.
ELISA 1/20000.


  • FunctionTranscription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. Promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. Involved in activated KRAS-mediated transcriptional activation of USP28 in colorectal cancer (CRC) cells (PubMed:24623306). Binds to the USP28 promoter in colorectal cancer (CRC) cells (PubMed:24623306).
  • Sequence similaritiesBelongs to the bZIP family. Jun subfamily.
    Contains 1 bZIP (basic-leucine zipper) domain.
  • Post-translational
    Ubiquitinated by the SCF(FBXW7), leading to its degradation. Ubiquitination takes place following phosphorylation, that promotes interaction with FBXW7.
    Phosphorylated by CaMK4 and PRKDC; phosphorylation enhances the transcriptional activity. Phosphorylated by HIPK3. Phosphorylated by DYRK2 at Ser-243; this primes the protein for subsequent phosphorylation by GSK3B at Thr-239. Phosphorylated at Thr-239, Ser-243 and Ser-249 by GSK3B; phosphorylation reduces its ability to bind DNA. Phosphorylated by PAK2 at Thr-2, Thr-8, Thr-89, Thr-93 and Thr-286 thereby promoting JUN-mediated cell proliferation and transformation. Phosphorylated by PLK3 following hypoxia or UV irradiation, leading to increase DNA-binding activity.
    Acetylated at Lys-271 by EP300.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Activator protein 1 antibody
    • AP 1 antibody
    • AP1 antibody
    • cJun antibody
    • Enhancer Binding Protein AP1 antibody
    • Jun Activation Domain Binding Protein antibody
    • JUN antibody
    • Jun oncogene antibody
    • JUN protein antibody
    • Jun proto oncogene antibody
    • JUN_HUMAN antibody
    • JUNC antibody
    • Oncogene JUN antibody
    • p39 antibody
    • Proto oncogene c jun antibody
    • Proto oncogene cJun antibody
    • Proto-oncogene c-jun antibody
    • Transcription Factor AP 1 antibody
    • Transcription factor AP-1 antibody
    • Transcription Factor AP1 antibody
    • V jun avian sarcoma virus 17 oncogene homolog antibody
    • V jun sarcoma virus 17 oncogene homolog (avian) antibody
    • V jun sarcoma virus 17 oncogene homolog antibody
    • V-jun avian sarcoma virus 17 oncogene homolog antibody
    • vJun Avian Sarcoma Virus 17 Oncogene Homolog antibody
    see all

Anti-c-Jun (phospho T91) antibody images

  • All lanes : Anti-c-Jun (phospho T91) antibody (ab28853) at 1/500 dilution

    Lane 1 : HeLa cell lysate -untreated
    Lane 2 : HeLa cell lysate -treated with UV light

    Predicted band size : 36 kDa
    Observed band size : 45 kDa (why is the actual band size different from the predicted?)
  • These images show human breast carcinoma stained with ab28853. In the right hand image the same antibody was preincubated with synthesized phosphopeptide.

References for Anti-c-Jun (phospho T91) antibody (ab28853)

This product has been referenced in:
  • Marek KW  et al. cJun integrates calcium activity and tlx3 expression to regulate neurotransmitter specification. Nat Neurosci 13:944-50 (2010). Read more (PubMed: 20581840) »

See 1 Publication for this product

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