Synthetic peptide corresponding to c-Jun (phospho T93). Immunogen is in the range of aa 61-110.
Synthesized phospho peptide derived from human c-Jun around the phosphorylation site of Threonine 93. Database link: P05412
IHC-P: human breast carcinoma
WB: HeLa cell extract.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Purification notesThe antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide.
The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Detects a band of approximately 45 kDa (predicted molecular weight: 36 kDa).
1/50 - 1/100.
Use at an assay dependent concentration.
FunctionTranscription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. Promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. Involved in activated KRAS-mediated transcriptional activation of USP28 in colorectal cancer (CRC) cells (PubMed:24623306). Binds to the USP28 promoter in colorectal cancer (CRC) cells (PubMed:24623306).
Sequence similaritiesBelongs to the bZIP family. Jun subfamily. Contains 1 bZIP (basic-leucine zipper) domain.
Post-translational modificationsUbiquitinated by the SCF(FBXW7), leading to its degradation. Ubiquitination takes place following phosphorylation, that promotes interaction with FBXW7. Phosphorylated by CaMK4 and PRKDC; phosphorylation enhances the transcriptional activity. Phosphorylated by HIPK3. Phosphorylated by DYRK2 at Ser-243; this primes the protein for subsequent phosphorylation by GSK3B at Thr-239. Phosphorylated at Thr-239, Ser-243 and Ser-249 by GSK3B; phosphorylation reduces its ability to bind DNA. Phosphorylated by PAK2 at Thr-2, Thr-8, Thr-89, Thr-93 and Thr-286 thereby promoting JUN-mediated cell proliferation and transformation. Phosphorylated by PLK3 following hypoxia or UV irradiation, leading to increase DNA-binding activity. Acetylated at Lys-271 by EP300.
Ab28854, at a dilution of 1/50, staining c-Jun in paraffin embedded human breast carcinoma tissue by Immunohistochemistry.
Left image: using ab28854.
Right image: using the same antibody preincubated with the synthesized phosphopeptide.Ab28854, at a dilution of 1/50, staining c-Jun in paraffin embedded human breast carcinoma tissue by Immunohistochemistry.
Left image: using ab28854.
Right image: using the same antibody preincubated with the synthesized phosphopeptide.
References for Anti-c-Jun (phospho T93) antibody (ab28854)
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