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The details of the immunogen for this antibody are not available.
Our Abpromise guarantee covers the use of ab25022 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||Use at an assay dependent concentration.|
|Flow Cyt||Use 1µg for 106 cells. ab18542-Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.|
|Functional Studies||Use at an assay dependent concentration. Can be used for cell homing studies, blockade of hematopoiesis and disruption fo melanocyte development.|
|ICC/IF||Use at an assay dependent concentration.|
Flow Cytometry analysis of C57BL/6 mouse bone marrow cells labeling c-Kit with ab25022 at 0.3 μg/106 cells dilution. Rat Anti-Mouse CD45R-PE followed by Streptavidin-FITC was used as the secondary antibody.