Overview

  • Product name
    Anti-c-Kit antibody [YR145]
    See all c-Kit primary antibodies
  • Description
    Rabbit monoclonal [YR145] to c-Kit
  • Specificity
    This antibody is specific to c-Kit. It does not crossreact with other CSF1/PDGF receptor family member.
  • Tested applications
    Suitable for: IHC-P, WBmore details
    Unsuitable for: Flow Cyt or ICC/IF
  • Species reactivity
    Reacts with: Human, Common marmoset
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human c-Kit aa 950 to the C-terminus. (cytoplasmic domain).

  • Positive control
    • WB: Human fetal kidney, fetal lung and seminoma tissue lysate. IHC-P: Human lung adenocarcinoma and seminoma tissues.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

     

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Applications

Our Abpromise guarantee covers the use of ab32363 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/400. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See protocols (link: http://www.abcam.com/protocols/ihc-antigen-retrieval-protocol).

WB 1/1000. Predicted molecular weight: 110 kDa.
  • Application notes
    Is unsuitable for Flow Cyt or ICC/IF.
  • Target

    • Function
      Tyrosine-protein kinase that acts as cell-surface receptor for the cytokine KITLG/SCF and plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. In response to KITLG/SCF binding, KIT can activate several signaling pathways. Phosphorylates PIK3R1, PLCG1, SH2B2/APS and CBL. Activates the AKT1 signaling pathway by phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase. Activated KIT also transmits signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. Promotes activation of STAT family members STAT1, STAT3, STAT5A and STAT5B. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KIT signaling is modulated by protein phosphatases, and by rapid internalization and degradation of the receptor. Activated KIT promotes phosphorylation of the protein phosphatases PTPN6/SHP-1 and PTPRU, and of the transcription factors STAT1, STAT3, STAT5A and STAT5B. Promotes phosphorylation of PIK3R1, CBL, CRK (isoform Crk-II), LYN, MAPK1/ERK2 and/or MAPK3/ERK1, PLCG1, SRC and SHC1.
    • Tissue specificity
      Isoform 1 and isoform 2 are detected in spermatogonia and Leydig cells. Isoform 3 is detected in round spermatids, elongating spermatids and spermatozoa (at protein level). Widely expressed. Detected in the hematopoietic system, the gastrointestinal system, in melanocytes and in germ cells.
    • Involvement in disease
      Piebald trait
      Gastrointestinal stromal tumor
      Testicular germ cell tumor
      Leukemia, acute myelogenous
    • Sequence similarities
      Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
      Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
      Contains 1 protein kinase domain.
    • Post-translational
      modifications
      Ubiquitinated by SOCS6. KIT is rapidly ubiquitinated after autophosphorylation induced by KITLG/SCF binding, leading to internalization and degradation.
      Autophosphorylated on tyrosine residues. KITLG/SCF binding enhances autophosphorylation. Isoform 1 shows low levels of tyrosine phosphorylation in the absence of added KITLG/SCF (in vitro). Kinase activity is down-regulated by phosphorylation on serine residues by protein kinase C family members. Phosphorylation at Tyr-568 is required for interaction with PTPN11/SHP-2, CRK (isoform Crk-II) and members of the SRC tyrosine-protein kinase family. Phosphorylation at Tyr-570 is required for interaction with PTPN6/SHP-1. Phosphorylation at Tyr-703, Tyr-823 and Tyr-936 is important for interaction with GRB2. Phosphorylation at Tyr-721 is important for interaction with PIK3R1. Phosphorylation at Tyr-823 and Tyr-936 is important for interaction with GRB7.
    • Cellular localization
      Cell membrane and Cytoplasm. Detected in the cytoplasm of spermatozoa, especially in the equatorial and subacrosomal region of the sperm head.
    • Information by UniProt
    • Database links
    • Alternative names
      • C Kit antibody
      • c-Kit antibody
      • c-Kit Ligand antibody
      • CD117 antibody
      • Kit antibody
      • Kit Ligand antibody
      • KIT oncogene antibody
      • KIT proto oncogene receptor tyrosine kinase antibody
      • KIT_HUMAN antibody
      • Mast cell growth factor receptor antibody
      • Mast/stem cell growth factor receptor Kit antibody
      • MGF antibody
      • p145 c-kit antibody
      • PBT antibody
      • Piebald trait protein antibody
      • Proto oncogene c Kit antibody
      • Proto oncogene tyrosine protein kinase Kit antibody
      • Proto-oncogene c-Kit antibody
      • SCF Receptor antibody
      • SCFR antibody
      • soluble KIT variant 1 antibody
      • Steel Factor Receptor antibody
      • Stem cell factor receptor antibody
      • tyrosine protein kinase Kit antibody
      • Tyrosine-protein kinase Kit antibody
      • v kit Hardy Zuckerman 4 feline sarcoma viral oncogene homolog antibody
      • v kit Hardy Zuckerman 4 feline sarcoma viral oncogene like protein antibody
      • v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog antibody
      see all

    Anti-c-Kit antibody [YR145] images

    • Anti-c-Kit antibody [YR145] (ab32363) at 1/5000 dilution (purified) + Human seminoma tissue lysate at 10 µg

      Secondary
      HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG at 1/50000 dilution

      Predicted band size : 110 kDa
      Observed band size : 120-145 kDa (why is the actual band size different from the predicted?)

      Blocking and dilution buffer: 5% NFDM/TBST.

    • Anti-c-Kit antibody [YR145] (ab32363) at 1/1000 dilution (purified) + Human fetal lung tissue lysate at 10 µg

      Secondary
      HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG at 1/50000 dilution

      Predicted band size : 110 kDa
      Observed band size : 120-145 kDa (why is the actual band size different from the predicted?)

      Blocking and dilution buffer: 5% NFDM/TBST.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human seminoma tissue labelling c-Kit with purified ab32363 at 1/400. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human glioma tissue labelling c-Kit with unpurified ab32363.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human seminoma tissue labelling c-Kit with unpurified ab32363.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling c-Kit with unpurified ab32363.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling c-Kit with unpurified ab32363.

    • IHC-P image of c-Kit (unpurified ab32363) staining on adult marmoset testis. The sections were fixed in formaldehyde and underwent heat mediated antigen retrieval, using Dako antigen retrieval solution. The sections were then blocked in 5% milk for 30 mins at 25°C. The primary antibody was added at a 1/100 dilution, and then incubated for 18 hours at 4°C. Dapi was used to stain the nuclei (blue).

      See Abreview

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References for Anti-c-Kit antibody [YR145] (ab32363)

    This product has been referenced in:
    • Yu-Ching Lin Z  et al. Gene expression ontogeny of spermatogenesis in the marmoset uncovers primate characteristics during testicular development. Dev Biol N/A:N/A (2015). IHC-P ; Common marmoset . Read more (PubMed: 25624265) »
    • Vajravelu BN  et al. C-Kit Promotes Growth and Migration of Human Cardiac Progenitor Cells via the PI3K-AKT and MEK-ERK Pathways. PLoS One 10:e0140798 (2015). WB ; Human . Read more (PubMed: 26474484) »

    See all 10 Publications for this product

    Product Wall

    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Dog Tissue sections (Atria)
    Permeabilization
    Yes - 0.3% TX-100,10mg/mL BSA, 5% Normal Goat Serum, in PBS
    Specification
    Atria
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Username

    Dr. Juan Estrada

    Verified customer

    Submitted Apr 05 2016

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Specification
    HeLa
    Permeabilization
    Yes - 0.5% Triton X100 in PBS
    Fixative
    Paraformaldehyde
    Username

    Dr. Kirk Mcmanus

    Verified customer

    Submitted Mar 27 2014

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Marmoset (common) Tissue sections (adult testis)
    Specification
    adult testis
    Fixative
    Formaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Dako antigen retrieval solution
    Permeabilization
    No
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    煜欽 林

    Verified customer

    Submitted Feb 06 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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