Overview

  • Product namec-Kit (CD117) Human ELISA Kit
  • Detection methodColorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Sample A 8 5.629ng/ml 0.34 6%
    Sample B 8 1.231ng/ml 0.06 4.8%
    Inter-assay
    Sample n Mean SD CV%
    Sample A 8 5.44ng/ml 0.43 7.9%
    Sample B 8 1.314ng/ml 0.1 7.6%
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 0.095 ng/ml
  • Range
    0.312 ng/ml - 10 ng/ml
  • Assay time
    1h 45m
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s c-Kit Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of c-Kit in Human serum, plasma, buffered solutions or supernatants.

    A monoclonal antibody specific for c-Kit has been coated onto the wells of the microtiter strips provided. Samples, including standards of known c-Kit concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for c-Kit are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of c-Kit present in the samples.

    This kit will recognize both endogenous and recombinant Human c-Kit.

  • Tested applicationsSandwich ELISA more details
  • PlatformMicroplate

Properties

  • RelevanceKIT encodes the human homolog of the proto-oncogene c-kit. c-kit was first identified as the cellular homolog of the feline sarcoma viral oncogene v-kit. KIT is a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). Mutations in KIT are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous lukemia, and piebaldism.
  • Cellular localizationCell Membrane; single-pass type I membrane protein
  • Alternative names
    • CD117
    • CD117 antigen
    • EC 2.7.10.1
    • Mast/stem cell growth factor receptor precursor
    • PBT
    • Proto oncogene tyrosine protein kinase Kit
    • SCFR
    • v kit Hardy Zuckerman 4 feline sarcoma viral oncogene homolog
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab45924 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
Sandwich ELISA Use at an assay dependent concentration.

c-Kit (CD117) Human ELISA Kit images

  • Representative Standard Curve using ab45924.

Protocols

References for c-Kit (CD117) Human ELISA Kit (ab45924)

ab45924 has not yet been referenced specifically in any publications.

Product Wall


The c-Kit Human ELISA Kit (ab45924) and Ferritin Human ELISA Kit (ab108698) both come with strip wells provided.

Thank you for your enquiry.

ab45925 is suitable for Cerebrospinal fluid.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"