Overview

  • Product name
  • Description
    Rabbit polyclonal to c-Myb
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Macaque monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 200 - 300 of Human c-Myb.

  • Positive control
    • This antibody gave a positive signal in both Human Spleen and Thymus tissue lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
    Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab109633 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 72 kDa (predicted molecular weight: 72 kDa).

Target

  • Function
    Transcriptional activator; DNA-binding protein that specifically recognize the sequence 5'-YAAC[GT]G-3'. Plays an important role in the control of proliferation and differentiation of hematopoietic progenitor cells.
  • Sequence similarities
    Contains 3 HTH myb-type DNA-binding domains.
  • Domain
    Comprised of 3 domains; an N-terminal DNA-binding domain, a centrally located transcriptional activation domain and a C-terminal domain involved in transcriptional repression.
  • Post-translational
    modifications
    Ubiquitinated; mediated by SIAH1 and leading to its subsequent proteasomal degradation.
    Phosphorylated by NLK on multiple sites, which induces proteasomal degradation.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • C myb antibody
    • c myb protein (140 AA) antibody
    • c-myb protein (140 AA) antibody
    • c-myb_CDS antibody
    • c-myb10A_CDS antibody
    • c-myb13A_CDS antibody
    • c-myb14A_CDS antibody
    • c-myb8B_CDS antibody
    • Cmyb antibody
    • efg antibody
    • Myb antibody
    • MYB proto-oncogene transcription factor antibody
    • MYB_HUMAN antibody
    • ONCOGENE AMV antibody
    • Oncogene MYB antibody
    • Proto oncogene c Myb antibody
    • Proto-oncogene c-Myb antibody
    • Transcriptional activator Myb antibody
    • v myb avian myeloblastosis viral oncogene homolog antibody
    • v-myb avian myeloblastosis viral oncogene homolog antibody
    see all

Images

  • All lanes : Anti-c-Myb antibody (ab109633) at 1 µg/ml

    Lane 1 : Human spleen tissue lysate - total protein (ab29699)
    Lane 2 : Human thymus tissue lysate - total protein (ab30146)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 72 kDa
    Observed band size : 72 kDa
    Additional bands at : 24 kDa,52 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 6 minutes

References

ab109633 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab109633.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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