Overview

  • Product name
  • Description
    Rabbit polyclonal to c-Myb
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, IPmore details
    Unsuitable for: WB
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Cow, Cynomolgus monkey, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide within Human c-Myb aa 175-225. The exact sequence is proprietary. (NP_005366.2)
    Sequence:

    GRTDNA IKNHWNSTMR RKVEQEGYLQ ESSKASQPAV ATSFQKNSHL MGFAQ


    Database link: P10242

  • Positive control
    • Human breast carcinoma tissue; MOLT4 whole cell lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate

    pH 7 - 8.
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab177510 was affinity purified using an epitope specific to c-Myb immobilized on solid support.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab177510 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP Use at 2-10 µg/mg of lysate.
  • Application notes
    Is unsuitable for WB.
  • Target

    • Function
      Transcriptional activator; DNA-binding protein that specifically recognize the sequence 5'-YAAC[GT]G-3'. Plays an important role in the control of proliferation and differentiation of hematopoietic progenitor cells.
    • Sequence similarities
      Contains 3 HTH myb-type DNA-binding domains.
    • Domain
      Comprised of 3 domains; an N-terminal DNA-binding domain, a centrally located transcriptional activation domain and a C-terminal domain involved in transcriptional repression.
    • Post-translational
      modifications
      Ubiquitinated; mediated by SIAH1 and leading to its subsequent proteasomal degradation.
      Phosphorylated by NLK on multiple sites, which induces proteasomal degradation.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • C myb antibody
      • c myb protein (140 AA) antibody
      • c-myb protein (140 AA) antibody
      • c-myb_CDS antibody
      • c-myb10A_CDS antibody
      • c-myb13A_CDS antibody
      • c-myb14A_CDS antibody
      • c-myb8B_CDS antibody
      • Cmyb antibody
      • efg antibody
      • Myb antibody
      • MYB proto-oncogene transcription factor antibody
      • MYB_HUMAN antibody
      • ONCOGENE AMV antibody
      • Oncogene MYB antibody
      • Proto oncogene c Myb antibody
      • Proto-oncogene c-Myb antibody
      • Transcriptional activator Myb antibody
      • v myb avian myeloblastosis viral oncogene homolog antibody
      • v-myb avian myeloblastosis viral oncogene homolog antibody
      see all

    Images

    • Detection of Human c-Myb by Western Blot of Immunoprecipitate.

      Lane 1: Whole cell lysate (1 mg for IP; 20% of IP loaded) from MOLT4 cells.

      Lane 2: Control IgG.

      ab177510 used for IP at 6 μg/mg lysate.

      ab177510 was used at 1 μg/ml for WB.

      Detection: Chemiluminescence with an exposure time of 10 seconds.

    • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human breast carcinoma tissue labeling c-Myb with ab177510 at 1µg/ml. Detection:DAB.

    References

    ab177510 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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