The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Use at 2-10 µg/mg of lysate.
Is unsuitable for WB.
Transcriptional activator; DNA-binding protein that specifically recognize the sequence 5'-YAAC[GT]G-3'. Plays an important role in the control of proliferation and differentiation of hematopoietic progenitor cells.
Contains 3 HTH myb-type DNA-binding domains.
Comprised of 3 domains; an N-terminal DNA-binding domain, a centrally located transcriptional activation domain and a C-terminal domain involved in transcriptional repression.
Ubiquitinated; mediated by SIAH1 and leading to its subsequent proteasomal degradation. Phosphorylated by NLK on multiple sites, which induces proteasomal degradation.