Overview

  • Product nameAnti-c-Myb antibody [EPR718(2)]
    See all c-Myb primary antibodies
  • Description
    Rabbit monoclonal [EPR718(2)] to c-Myb
  • Tested applicationsSuitable for: IHC-P, WBmore details
    Unsuitable for: Flow Cyt,ICC or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic peptide corresponding to residues in Human c-Myb.

  • Positive control
    • Jurkat and MOLT4 cell lysates; Human colon tissue
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage bufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • PurityTissue culture supernatant
  • ClonalityMonoclonal
  • Clone numberEPR718(2)
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab109127 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. PubMed: 22569827
WB 1/1000 - 1/10000. Predicted molecular weight: 72 kDa.
  • Application notesIs unsuitable for Flow Cyt,ICC or IP.
  • Target

    • FunctionTranscriptional activator; DNA-binding protein that specifically recognize the sequence 5'-YAAC[GT]G-3'. Plays an important role in the control of proliferation and differentiation of hematopoietic progenitor cells.
    • Sequence similaritiesContains 3 HTH myb-type DNA-binding domains.
    • DomainComprised of 3 domains; an N-terminal DNA-binding domain, a centrally located transcriptional activation domain and a C-terminal domain involved in transcriptional repression.
    • Post-translational
      modifications
      Ubiquitinated; mediated by SIAH1 and leading to its subsequent proteasomal degradation.
      Phosphorylated by NLK on multiple sites, which induces proteasomal degradation.
    • Cellular localizationNucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • C myb antibody
      • c myb protein (140 AA) antibody
      • c-myb protein (140 AA) antibody
      • c-myb_CDS antibody
      • c-myb10A_CDS antibody
      • c-myb13A_CDS antibody
      • c-myb14A_CDS antibody
      • c-myb8B_CDS antibody
      • Cmyb antibody
      • efg antibody
      • Myb antibody
      • MYB_HUMAN antibody
      • ONCOGENE AMV antibody
      • Oncogene MYB antibody
      • Proto oncogene c Myb antibody
      • Proto-oncogene c-Myb antibody
      • Transcriptional activator Myb antibody
      • v myb avian myeloblastosis viral oncogene homolog antibody
      • v-myb avian myeloblastosis viral oncogene homolog antibody
      see all

    Anti-c-Myb antibody [EPR718(2)] images

    • All lanes : Anti-c-Myb antibody [EPR718(2)] (ab109127) at 1/1000 dilution

      Lane 1 : Jurkat cell lysates
      Lane 2 : MOLT4 cell lysates

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 72 kDa

    References for Anti-c-Myb antibody [EPR718(2)] (ab109127)

    This product has been referenced in:
    • Cyr AR  et al. TFAP2C governs the luminal epithelial phenotype in mammary development and carcinogenesis. Oncogene N/A:N/A (2014). Read more (PubMed: 24469049) »
    • Baron BW  et al. GFI1B, EVI5, MYB-Additional genes that cooperate with the human BCL6 gene to promote the development of lymphomas. Blood Cells Mol Dis N/A:N/A (2013). IHC . Read more (PubMed: 23910958) »

    See all 3 Publications for this product

    Product Wall

    There are currently no Abreviews or Questions for ab109127.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"