Purification notesab111339 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
FunctionTranscriptional activator; DNA-binding protein that specifically recognize the sequence 5'-YAAC[GT]G-3'. Plays an important role in the control of proliferation and differentiation of hematopoietic progenitor cells.
Immunohistochemistry analysis of paraffin-embedded Human breast carcinoma tissue, using ab111339 at a dilution of 1/50. Left panel shows staining without immunizing peptide right panel shows staining with immunizing peptide.
References for Anti-c-Myb (phospho S532) antibody (ab111339)
has not yet been referenced specifically in any publications.
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