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Synthetic peptide: AEEQKLISEE DLLRKRREQL KHKLE conjugated to KLH, corresponding to C terminal amino acids 408-432 of Human c-Myc.
Our Abpromise guarantee covers the use of ab32 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 5 µg/ml.|
|ChIP||Use at an assay dependent concentration.|
|IHC (Methanol fixed)||1/200. PubMed: 17329357|
|Flow Cyt||Use 1µg for 106 cells. Ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.|
|WB||1/500 - 1/1000. This antibody has been used for immunolocalisation with gold conjugated secondaries on plant tissue.|
|IP||Use at 6 µg/mg of lysate. It is not known whether this antibody is suitable for immunoprecipitation of native c-myc protein.|
|ELISA||Use at an assay dependent concentration.|
|IHC-P||1/250 - 1/500.|
|IHC-Fr||1/1000. See Abreviews.|
|Purification||Use at an assay dependent concentration.|
Menssen R et al., (2001) Curr Biol. Mar 6;11(5):345-50.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"