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Synthetic peptide: AEEQKLISEE DLLRKRREQL KHKLE conjugated to KLH, corresponding to C terminal amino acids 408-432 of Human c-Myc.
myc is involved in MAPK-p37 signaling pathway. If you need conjugated c-Myc (9E10) antibodies, find our range of products here.
Our Abpromise guarantee covers the use of ab32 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 5 µg/ml.|
|ChIP||Use at an assay dependent concentration.|
|IHC (Methanol fixed)||1/200. PubMed: 17329357|
|Flow Cyt||Use 1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|WB||1/500 - 1/1000. This antibody has been used for immunolocalisation with gold conjugated secondaries on plant tissue.|
|IP||Use at 6 µg/mg of lysate. It is not known whether this antibody is suitable for immunoprecipitation of native c-myc protein.|
|ELISA||Use at an assay dependent concentration.|
|IHC-P||1/250 - 1/500.|
|IHC-Fr||1/1000. See Abreviews.|
|Purification||Use at an assay dependent concentration.|
Menssen R et al., (2001) Curr Biol. Mar 6;11(5):345-50.
Phosphorylation of Cdc15 changes during the cell cycle. Exponentially growing cells (cyc) of CDC15-MYC9 (W1114) were arrested in G1 with a factor pheromone and released into fresh medium at 25°C. Cells were harvested at the indicated times, the percentage of divided nuclei was determined by DAPI staining of fixed cells, and proteins were analyzed by western blotting with 9E10 (ab32).
ab32 staining c-Myc in human esophagus tissue sections by immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 10% serum for 1 hour at 20°C; antigen retrieval was by heat mediation in a citric buffer. Samples were incubated with primary antibody (1/300) for 12 hours at 4°C. A HRP-conjugated rabbit anti-mouse polyclonal (1/200) was used as the secondary antibody.
ab32 staining c-Myc in HEK293 cells transfected with pCMV4-myc-Mfn2 by immunocytochemistry/ immunofluorescence.
Cells were fixed with paraformaldehyde, permeabilized with 1% Triton X-100 and blocked with 5% horse serum for 1 hour at room temperature. Samples were incubated with primary antibody (1/1200 in 5% horse serum) for 1 hour. An AlexaFluor®488-conjugated goat anti-mouse polyclonal IgG (1/400) was used as the secondary antibody.