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Anti-c-Myc antibody [9E11] - ChIP Grade (ab56)

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Overview

Product name

Anti-c-Myc antibody [9E11] - ChIP Grade
See all c-Myc products (42) ...

Description

Mouse monoclonal [9E11] to c-Myc - ChIP Grade

Conjugation notes

-

Tested applications

Flow Cyt, ChIP, WB, IP, IHC-P, IHC-Frmore details

Cross reactivity

Reacts with

Chicken, Human, Saccharomyces cerevisiae

Immunogen

Synthetic peptide: AEEQKLISEEDL conjugated to KLH, corresponding to amino acids 408-420 of Human c-Myc.

Positive control

HL-60 cells or breast tumors.

General notes

-

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Phosphate buffered saline

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Clonality

Monoclonal

Clone number

9E11

Myeloma

Sp2

Isotype

IgG2a

Light chain type

kappa

Research areas

Epigenetics and Nuclear Signaling >> ChIP'ing antibodies >> ChIP'ing antibodies
Cancer >> Tumor biomarkers >> Oncoproteins
Cancer >> Oncoproteins/suppressors >> Oncoproteins >> Transcription factors
Cancer >> Cell cycle >> Cell differentiation
Epigenetics and Nuclear Signaling >> Transcription >> Transcription Factors
Stem Cells >> Signaling Pathways >> TGF beta >> Nuclear
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> HLH / Leucine Zipper

  • Flow Cytometry - c-Myc antibody [9E11] - ChIP Grade (ab56)Flow Cytometry - c-Myc antibody [9E11] - ChIP Grade (ab56) image (enlarge)

  • Western blot - c-Myc antibody [9E11] - ChIP Grade (ab56)Western blot - c-Myc antibody [9E11] - ChIP Grade (ab56) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab56 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Function

Participates in the regulation of gene transcription. Binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Seems to activate the transcription of growth-related genes.

Involvement in disease

Note=Overexpression of MYC is implicated in the etiology of a variety of hematopoietic tumors.
Note=A chromosomal aberration involving MYC may be a cause of a form of B-cell chronic lymphocytic leukemia. Translocation t(8;12)(q24;q22) with BTG1.
Defects in MYC are a cause of Burkitt lymphoma (BL) [MIM:113970]. A form of undifferentiated malignant lymphoma commonly manifested as a large osteolytic lesion in the jaw or as an abdominal mass. Note=Chromosomal aberrations involving MYC are usually found in Burkitt lymphoma. Translocations t(8;14), t(8;22) or t(2;8) which juxtapose MYC to one of the heavy or light chain immunoglobulin gene loci.

Sequence similarities

Contains 1 basic helix-loop-helix (bHLH) domain.

Post-translational
modifications

Phosphorylated by PRKDC. Phosphorylation at Thr-58 and Ser-62 by GSK3 is required for ubiquitination and degradation by the proteasome.
Ubiquitinated by the SCF(FBXW7) complex when phosphorylated at Thr-58 and Ser-62, leading to its degradation by the proteasome. In the nucleoplasm, ubiquitination is counteracted by USP28, which interacts with isoform 1 of FBXW7 (FBW7alpha), leading to its deubiquitination and preventing degradation. In the nucleolus, however, ubiquitination is not counteracted by USP28, due to the lack of interaction between isoform 4 of FBXW7 (FBW7gamma) and USP28, explaining the selective MYC degradation in the nucleolus. Also polyubiquitinated by the DCX(TRUSS) complex.

Cellular localization

Nucleus > nucleoplasm. Nucleus > nucleolus.

Target information above from: UniProt accessionP01106 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • AU016757 antibody
  • Avian myelocytomatosis viral oncogene homolog antibody
  • bHLHe39 antibody
  • c Myc antibody
  • Cellular myelocytomatosis oncogene antibody
  • Class E basic helix-loop-helix protein 39 antibody
  • MGC105490 antibody
  • MRTL antibody
  • Myc antibody
  • Myc protein antibody
  • Myc proto oncogene protein antibody
  • Myc proto-oncogene protein antibody
  • Myc-related translation/localization regulatory factor antibody
  • MYC_HUMAN antibody
  • Myc2 antibody
  • myca antibody
  • Myelocytomatosis oncogene a antibody
  • Myelocytomatosis oncogene antibody
  • Niard antibody
  • Nird antibody
  • Oncogene Myc antibody
  • OTTHUMP00000158589 antibody
  • OTTHUMP00000227763 antibody
  • Proto-oncogene c-Myc antibody
  • Protooncogene homologous to myelocytomatosis virus antibody
  • RNCMYC antibody
  • Transcription factor p64 antibody
  • Transcriptional regulator Myc-A antibody
  • v myc avian myelocytomatosis viral oncogene homolog antibody
  • v myc myelocytomatosis viral oncogene homolog (avian) antibody
  • V-Myc avian myelocytomatosis viral oncogene homolog antibody
  • v-myc myelocytomatosis viral oncogene homolog (avian) antibody
  • zc-myc antibody
see all

Database links

Anti-c-Myc antibody [9E11] - ChIP Grade images:

  Flow Cytometry - c-Myc antibody [9E11] - ChIP Grade (ab56)

Flow Cytometry - c-Myc antibody [9E11] - ChIP Grade (ab56)

Overlay histogram showing HL60 cells stained with ab56 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab56, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

  Western blot - c-Myc antibody [9E11] - ChIP Grade (ab56)

Western blot - c-Myc antibody [9E11] - ChIP Grade (ab56)

Anti-c-Myc antibody [9E11] - ChIP Grade (ab56) at 1/1000 dilution + whole cell lysate prepared from huh-7 cells at 15 µg

Secondary
Anti-mouse IgG conjugated to HRP at 1/10000 dilution

Predicted band size : 49 kDa

Image courtesy of an anonymous Abreview.

References for Anti-c-Myc antibody [9E11] - ChIP Grade (ab56)

This product has been referenced in:

  • Lian HYet al. The effect of Ku on telomere replication time is mediated by telomere length but is independent of histone tail acetylation. Mol Biol Cell 22:1753-65 (2011).Read more (PubMed: 21441303) »
  • Mankame TPet al. Identification and characterization of the human NOL7 gene promoter. Gene 456:36-44 (2010).Read more (PubMed: 20206243) »

See all 13 publications for this product

Publishing research using ab56? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"