Anti-Myc tag antibody (ab152146)
Key features and details
- Rabbit polyclonal to Myc tag
- Suitable for: WB, IP, ICC/IF
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Myc tag antibody
See all Myc tag primary antibodies -
Description
Rabbit polyclonal to Myc tag -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human Myc tag aa 400 to the C-terminus. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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Positive control
- 293T cells transfected with Myc tag recombinant protein lysate; HeLa Myc tag transfected lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Assay kits
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab152146 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/5000 - 1/20000.
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IP |
1/10000.
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ICC/IF |
1/100 - 1/1000.
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Notes |
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WB
1/5000 - 1/20000. |
IP
1/10000. |
ICC/IF
1/100 - 1/1000. |
Target
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Relevance
Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells. -
Cellular localization
Nuclear -
Database links
- Entrez Gene: 4609 Human
- Omim: 190080 Human
- SwissProt: P01106 Human
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Alternative names
- c-myc tag antibody
- Myc Epitope Tag antibody
Images
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All lanes : Anti-Myc tag antibody (ab152146) at 1/5000 dilution
Lane 1 : Non-transfected 293T cell lysate
Lane 2 : Transfected 293T cell lysate
Lysates/proteins at 10 µg per lane. -
Immunofluorescence analysis of paraformaldehyde fixed HeLa Myc tag transfected cells, labeling Myc tag using ab152146 at 1/2500 dilution (green) and alpha tubulin filaments (red).
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Myc antibody Immunoprecipitation of Myc-tagged protein. HeLa transfected lysates were subjected to immunoprecipitation using:
1: Input, 15ug of HeLa transfected lysates.
2: normal rabbit IgG or
3: 2.5 ug of ab152146.
The precipitated protein was detected by ab152146 diluted at 1/10000.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (9)
ab152146 has been referenced in 9 publications.
- Balogh D et al. Listeria monocytogenes utilizes the ClpP1/2 proteolytic machinery for fine-tuned substrate degradation at elevated temperatures. RSC Chem Biol 3:955-971 (2022). PubMed: 35866172
- Zhao L et al. Circ_0018168 inhibits the proliferation and osteogenic differentiation of fibroblasts in ankylosing spondylitis via regulating miR-330-3p/DKK1 axis. Regen Ther 21:175-184 (2022). PubMed: 35891711
- Fan Q et al. HPV-16/18 E6-induced APOBEC3B expression associates with proliferation of cervical cancer cells and hypomethylation of Cyclin D1. Mol Carcinog 60:313-330 (2021). PubMed: 33631046
- Chaudhary S et al. Dual blockade of EGFR and CDK4/6 delays head and neck squamous cell carcinoma progression by inducing metabolic rewiring. Cancer Lett 510:79-92 (2021). PubMed: 33878394
- Wu MJ et al. Targeting KDM4B that coactivates c-Myc-regulated metabolism to suppress tumor growth in castration-resistant prostate cancer. Theranostics 11:7779-7796 (2021). PubMed: 34335964
- Hu Y et al. m6A RNA Methylation Regulator YTHDF1 Correlated With Immune Microenvironment Predicts Clinical Outcomes and Therapeutic Efficacy in Breast Cancer. Front Med (Lausanne) 8:667543 (2021). PubMed: 34434939
- Chen H et al. LINC01094 promotes the invasion of ovarian cancer cells and regulates the Wnt/β-catenin signaling pathway by targeting miR-532-3p. Exp Ther Med 22:1228 (2021). PubMed: 34539824
- Wang J et al. MicroRNA-520b Suppresses Proliferation, Migration, and Invasion of Spinal Osteosarcoma Cells via Downregulation of Frizzled-8. Oncol Res 25:1297-1304 (2017). PubMed: 28247840
- Ji S et al. YEATS Domain Containing 4 Promotes Gastric Cancer Cell Proliferation and Mediates Tumor Progression via Activating the Wnt/ß-Catenin Signaling Pathway. Oncol Res 25:1633-1641 (2017). PubMed: 28251887