Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab28842 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
ELISA 1/1000.
ICC/IF Use a concentration of 1 µg/ml.
WB 1/500 - 1/1000. Predicted molecular weight: 49 kDa.

Target

  • FunctionParticipates in the regulation of gene transcription. Binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Seems to activate the transcription of growth-related genes.
  • Involvement in diseaseNote=Overexpression of MYC is implicated in the etiology of a variety of hematopoietic tumors.
    Note=A chromosomal aberration involving MYC may be a cause of a form of B-cell chronic lymphocytic leukemia. Translocation t(8;12)(q24;q22) with BTG1.
    Defects in MYC are a cause of Burkitt lymphoma (BL) [MIM:113970]. A form of undifferentiated malignant lymphoma commonly manifested as a large osteolytic lesion in the jaw or as an abdominal mass. Note=Chromosomal aberrations involving MYC are usually found in Burkitt lymphoma. Translocations t(8;14), t(8;22) or t(2;8) which juxtapose MYC to one of the heavy or light chain immunoglobulin gene loci.
  • Sequence similaritiesContains 1 basic helix-loop-helix (bHLH) domain.
  • Post-translational
    modifications
    Phosphorylated by PRKDC. Phosphorylation at Thr-58 and Ser-62 by GSK3 is required for ubiquitination and degradation by the proteasome. Phosphorylation at Ser-329 by PIM2 leads to the stabilization of MYC (By similarity). Phosphorylation at Ser-62 by CDK2 prevents Ras-induced senescence.
    Ubiquitinated by the SCF(FBXW7) complex when phosphorylated at Thr-58 and Ser-62, leading to its degradation by the proteasome. In the nucleoplasm, ubiquitination is counteracted by USP28, which interacts with isoform 1 of FBXW7 (FBW7alpha), leading to its deubiquitination and preventing degradation. In the nucleolus, however, ubiquitination is not counteracted by USP28, due to the lack of interaction between isoform 4 of FBXW7 (FBW7gamma) and USP28, explaining the selective MYC degradation in the nucleolus. Also polyubiquitinated by the DCX(TRUSS) complex.
  • Cellular localizationNucleus > nucleoplasm. Nucleus > nucleolus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Avian myelocytomatosis viral oncogene homolog antibody
    • bHLHe39 antibody
    • c Myc antibody
    • Class E basic helix-loop-helix protein 39 antibody
    • MRTL antibody
    • Myc antibody
    • Myc protein antibody
    • Myc proto oncogene protein antibody
    • Myc proto-oncogene protein antibody
    • myc related translation/localization regulatory factor antibody
    • MYC_HUMAN antibody
    • Myc2 antibody
    • MYCC antibody
    • Niard antibody
    • Nird antibody
    • Proto-oncogene c-Myc antibody
    • Transcription factor p64 antibody
    • v myc avian myelocytomatosis viral oncogene homolog antibody
    • v myc myelocytomatosis viral oncogene homolog antibody
    see all

Anti-c-Myc (phospho T58) antibody images

  • Immunohistochemical analysis of paraffin-embedded breast carcinoma. Left: Using Myc (Phospho-Thr58) Antibody; Right: The same antibody preincubated with synthesized phosphopeptide.
  • Lane 1 : Anti-c-Myc (phospho T58) antibody (ab28842) at 1/500 dilution
    Lane 2 : Anti-c-Myc (phospho T58) antibody (ab28842) at 1/500 dilution ( preincubated with synthesized non- phosphopeptide )
    Lane 3 : Anti-c-Myc (phospho T58) antibody (ab28842) at 1/500 dilution ( preincubated with synthesized phosphopeptide )

    Lane 1 : human ovarian cancer cell lysate
    Lane 2 : human ovarian cancer cell lysate
    Lane 3 : human ovarian cancer cell lysate


    Predicted band size : 49 kDa
    Observed band size : 51 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 120 kDa,55 kDa,86 kDa. We are unsure as to the identity of these extra bands.
  • ICC/IF image of ab28864 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28864, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-c-Myc (phospho T58) antibody (ab28842)

This product has been referenced in:
  • Chayka O  et al. Identification and pharmacological inactivation of the MYCN gene network as a therapeutic strategy for neuroblastic tumor cells. J Biol Chem 290:2198-212 (2015). WB ; Human . Read more (PubMed: 25477524) »
  • Xiao L  et al. Targeting Epstein-Barr virus oncoprotein LMP1-mediated glycolysis sensitizes nasopharyngeal carcinoma to radiation therapy. Oncogene N/A:N/A (2014). Human . Read more (PubMed: 24662831) »

See all 6 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing
Sample Human Cell lysate - whole cell (AGS)
Specification AGS
Blocking step BSA as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 3µg/mL · Temperature: 25°C
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Submitted Aug 15 2013

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