Overview

  • Product nameAnti-C Peptide antibody [1H8]
    See all C Peptide primary antibodies
  • Description
    Mouse monoclonal [1H8] to C Peptide
  • Specificityab8297 binds to the human free C-peptide and C-peptide region in proinsulin molecules. No cross reactivity with human, bovine, porcine insulins. This antibody detects a different epitope than the monoclonal, ab8298. Affinity (Kd) > 1 x 10^-8
  • Tested applicationsSuitable for: ICC/IF, IHC-P, Competitive ELISA, ELISA, RIAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein.

  • EpitopeThe three-dimensional structure generated by N- and C- terminal regions of C-peptide separated by beta-turn at position 47-50 of proinsulin.
  • General notes


    C Peptide is part of the molecule of Proinsulin, that consists of three parts: C Peptide and two long strands of amino acids (called the alpha and beta chains) that later become linked together to form the insulin molecule. From every molecule of proinsulin, one molecule of insulin plus one molecule of C Peptide are produced. C peptide is released into the blood stream in equal amounts to insulin. A test of C peptide levels will show how much insulin the body is making. Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPBS with 0.1% sodium azide, pH 7.4
  • Concentration information loading...
  • PurityProtein A purified
  • Primary antibody notesC Peptide is part of the molecule of Proinsulin, that consists of three parts: C Peptide and two long strands of amino acids (called the alpha and beta chains) that later become linked together to form the insulin molecule. From every molecule of proinsulin, one molecule of insulin plus one molecule of C Peptide are produced. C peptide is released into the blood stream in equal amounts to insulin. A test of C peptide levels will show how much insulin the body is making. Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.
  • ClonalityMonoclonal
  • Clone number1H8
  • Myelomax63-Ag8.653
  • IsotypeIgG1
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab8297 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
IHC-P Use a concentration of 1 µg/ml.
Competitive ELISA Use at an assay dependent concentration. Suitable for detection of C-peptide in human serum after immunospecific removal of proinsulin (using solid-phased Mouse monoclonal [1D6] to Proinsulin (ab8299).
ELISA Use at an assay dependent concentration.
RIA Use at an assay dependent concentration.

Target

  • FunctionInsulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.
  • Involvement in diseaseDefects in INS are the cause of familial hyperproinsulinemia (FHPRI) [MIM:176730].
    Defects in INS are a cause of diabetes mellitus insulin-dependent type 2 (IDDM2) [MIM:125852]. IDDM2 is a multifactorial disorder of glucose homeostasis that is characterized by susceptibility to ketoacidosis in the absence of insulin therapy. Clinical fetaures are polydipsia, polyphagia and polyuria which result from hyperglycemia-induced osmotic diuresis and secondary thirst. These derangements result in long-term complications that affect the eyes, kidneys, nerves, and blood vessels.
    Defects in INS are a cause of diabetes mellitus permanent neonatal (PNDM) [MIM:606176]. PNDM is a rare form of diabetes distinct from childhood-onset autoimmune diabetes mellitus type 1. It is characterized by insulin-requiring hyperglycemia that is diagnosed within the first months of life. Permanent neonatal diabetes requires lifelong therapy.
    Defects in INS are a cause of maturity-onset diabetes of the young type 10 (MODY10) [MIM:613370]. MODY10 is a form of diabetes that is characterized by an autosomal dominant mode of inheritance, onset in childhood or early adulthood (usually before 25 years of age), a primary defect in insulin secretion and frequent insulin-independence at the beginning of the disease.
  • Sequence similaritiesBelongs to the insulin family.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • IDDM 2 antibody
    • IDDM2 antibody
    • ILPR antibody
    • ins antibody
    • INS_HUMAN antibody
    • Insulin A chain antibody
    • IRDN antibody
    • MODY10 antibody
    • Proinsulin antibody
    see all

Anti-C Peptide antibody [1H8] images

  • ab8297 (1µg/ml) staining C Peptide in human pancreas, using an automated system (DAKO Autostainer Plus). Using this protocol there is strong cytoplasmic staining of the cells of the islets of Langerhans.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer citrate pH6.1 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
  • ICC/IF image of ab8297 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab8297, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-C Peptide antibody [1H8] (ab8297)

This product has been referenced in:
  • Yosten GL  et al. Evidence for an interaction between proinsulin C-peptide and GPR146. J Endocrinol 218:B1-8 (2013). ICC/IF . Read more (PubMed: 23759446) »
  • Masuda Y  et al. At pharmacologically relevant concentrations intravenous iron preparations cause pancreatic beta cell death. Am J Transl Res 6:64-70 (2013). ICC/IF ; Rat . Read more (PubMed: 24349622) »

See all 2 Publications for this product

Product Wall

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.
I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has c...

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I am sorry that my email wasn't clear enough. Lab has not performed any standard curve with this protein so they are unable to help.
My suggestions would be trying a different lot of one antibody to check the compatibility of protein with antibody...

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I agree the antibodies should detect the proteins in serum; as this is not the case so I am happy to replace these products. Would you like try new vials of these antibodies or a different antibody against the same target?
My suggestion would be tr...

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Thank you very much for confirming details.
The protein ab93903 has not been tested in ELISA and in combination with ab8297, ab8298 and ab48303 so we unfortunately do not hold any ELISA data for this product. This product was in fact used as blocki...

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Thank you for your email. I am sorry to hear that you have been experiencing problems with this antibody
.
I have read the details you have kindly provided and have following further questions for better understanding of the problem;
- Coul...

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Gracias por escribirnos.

Si lo que quiere la clienta es un anticuerpo contra C peptide para ICC que reaccione en humano, existen varias opciones.

Si lo que requiere además es que no reaccione con rata ni ratón me temo ...

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Thank you for your inquiry.

For ab8297, we have never tested this antibody for cross-reactivity with mouse or rat. Therefore, we would not be able to guarantee it for non-cross-reactivity with mouse and rat.

For ab108326, this antib...

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Thank you very much for contacting us.

It is my pleasure to send you the CoC for ab8297, lot number GR3913-4 (attached).

I hope this is helpful.

Have a lovely weekend! :-)

Unfortunately we have not tested the antibodies in this application, so we can't offer any recommendations.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"