Anti-C Peptide antibody [5B8] (ab1973)

Overview

  • Product nameAnti-C Peptide antibody [5B8]
    See all C Peptide primary antibodies
  • Description
    Mouse monoclonal [5B8] to C Peptide
  • SpecificityThis antibody binds with equal efficiency to the human free C-peptide and C-peptide region in proinsulin molecules. Antigenic determinants identified by the antibody are in the three-dimensional structure generated by N- and C-terminal regions of C-peptide separated by beta-turn at position 47-50 of proinsulin. Affinity (Kd) > 1 x 10^-8 This antibody does not cross-react with human, bovine, porcine and mouse/rat insulin.
  • Tested applicationsSuitable for: ELISA, RIA, Sandwich ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein.

  • Positive control
    • IHC-P (FFPE): Human Pancreas (Normal) tissue.
  • General notesConcentration varies from lot to lot and can be provided on request. Construction of a sensitive two-site sandwich EIA/ RIA immunosorbent assay for the measurement of total human proinsulin (including proinsulin convertion intermediates) in serum.


    C Peptide is part of the molecule of Proinsulin, that consists of three parts: C Peptide and two long strands of amino acids (called the alpha and beta chains) that later become linked together to form the insulin molecule. From every molecule of proinsulin, one molecule of insulin plus one molecule of C Peptide are produced. C peptide is released into the blood stream in equal amounts to insulin. A test of C peptide levels will show how much insulin the body is making. Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • PurityProtein A purified
  • Purification notesPurity is tested by electrophoresis.
  • Primary antibody notesC Peptide is part of the molecule of Proinsulin, that consists of three parts: C Peptide and two long strands of amino acids (called the alpha and beta chains) that later become linked together to form the insulin molecule. From every molecule of proinsulin, one molecule of insulin plus one molecule of C Peptide are produced. C peptide is released into the blood stream in equal amounts to insulin. A test of C peptide levels will show how much insulin the body is making. Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.
  • ClonalityMonoclonal
  • Clone number5B8
  • Myelomax63-Ag8.653
  • IsotypeIgG1
  • Light chain typekappa
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab1973 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration. Can be paired for ELISA with Mouse monoclonal [7E10] to C Peptide (ab1974).
RIA Use at an assay dependent concentration.
Sandwich ELISA Use at an assay dependent concentration. Construction of a sensitive two-site sandwich EIA/ RIA immunosorbent assay for the measurement of total human proinsulin (including proinsulin convertion intermediates) in serum. Can be used as detection antibody in sandwich ELISA with Mouse monoclonal [7E10] to C Peptide (ab1974) as capture antibody.
IHC-P Use a concentration of 0.1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionInsulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.
  • Involvement in diseaseDefects in INS are the cause of familial hyperproinsulinemia (FHPRI) [MIM:176730].
    Defects in INS are a cause of diabetes mellitus insulin-dependent type 2 (IDDM2) [MIM:125852]. IDDM2 is a multifactorial disorder of glucose homeostasis that is characterized by susceptibility to ketoacidosis in the absence of insulin therapy. Clinical fetaures are polydipsia, polyphagia and polyuria which result from hyperglycemia-induced osmotic diuresis and secondary thirst. These derangements result in long-term complications that affect the eyes, kidneys, nerves, and blood vessels.
    Defects in INS are a cause of diabetes mellitus permanent neonatal (PNDM) [MIM:606176]. PNDM is a rare form of diabetes distinct from childhood-onset autoimmune diabetes mellitus type 1. It is characterized by insulin-requiring hyperglycemia that is diagnosed within the first months of life. Permanent neonatal diabetes requires lifelong therapy.
    Defects in INS are a cause of maturity-onset diabetes of the young type 10 (MODY10) [MIM:613370]. MODY10 is a form of diabetes that is characterized by an autosomal dominant mode of inheritance, onset in childhood or early adulthood (usually before 25 years of age), a primary defect in insulin secretion and frequent insulin-independence at the beginning of the disease.
  • Sequence similaritiesBelongs to the insulin family.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • IDDM 2 antibody
    • IDDM2 antibody
    • ILPR antibody
    • ins antibody
    • INS_HUMAN antibody
    • Insulin A chain antibody
    • IRDN antibody
    • MODY10 antibody
    • Proinsulin antibody
    see all

Anti-C Peptide antibody [5B8] images

  • IHC image of C Peptide staining in Human Pancreas formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab1973, 0.1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

References for Anti-C Peptide antibody [5B8] (ab1973)

ab1973 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your enquiry.

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Thank you for contacting us. After reviewing the datasheets carefully I can confirm that the ab1974 and ab1973 antibodies can be used as a sandwich pair in sELISA. These are fully guaranteed to work and gave best results in QC experiment. Please be adv...

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Thanks again for your patience. I have heard back from the lab and each of the antibodies independently has been tested with I125-labelled C peptide, native or recombinant I125-labelled human proinsulin, and recombinant C peptide-streptavidin fu...

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I apologize for the extended delay getting back to you about this. We do not sell a purified C peptide, and I searched on other companies' websites but have not been able to find one. I contacted the production lab earlier and asked what they u...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"