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C Peptide is part of the molecule of Proinsulin, that consists of three parts: C Peptide and two long strands of amino acids (called the alpha and beta chains) that later become linked together to form the insulin molecule. From every molecule of proinsulin, one molecule of insulin plus one molecule of C Peptide are produced. C peptide is released into the blood stream in equal amounts to insulin. A test of C peptide levels will show how much insulin the body is making. Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.
Our Abpromise guarantee covers the use of ab1974 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent dilution. Can be paired for ELISA with Mouse monoclonal [5B8] to C Peptide (ab1973).|
|RIA||Use at an assay dependent dilution.|
|Sandwich ELISA||Use at an assay dependent dilution. Construction of a sensitive two-site sandwich EIA/ RIA immunosorbent assay for the measurement of total human proinsulin (including proinsulin convertion intermediates) in serum. Can be paired for sELISA as capture antibody with Mouse monoclonal [5B8] to C Peptide (ab1973) as detection antibody.|
ab1974 has not yet been referenced specifically in any publications.