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Abcam’s C-peptide Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of C-peptide in serum and plasma.
A 96-well plate has been precoated with Streptavidin. Samples, standards and the C-peptide HRP and Biotin conjugate are added to the wells. Biotinylated monoclonal and horseradish peroxidase (HRP) labelled antibodies are added and the reactants are mixed. The different types of antibodies used have high affinity and specificity and are directed against distinct and different epitopes of C-Peptide. Reaction between the various C-Peptide antibodies and native C-Peptide occurs in the microwells without competition or steric hindrance forming a soluble sandwich complex. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is directly proportional to the amount of C-peptide in the sample and the intensity is measured at 450 nm.
C-peptide is the abbreviation for connecting peptide; it is a 31-amminoacid peptide. C-peptide of insulin is the C-terminal cleavage product produced during processing of the insulin pro-hormone to the mature insulin molecule. Proinsulin is cleaved when it is released from the pancreas into the blood - one C-peptide for each insulin molecule. C-Peptide is devoid of any biological activity but appears to be necessary to maintain the structural integrity of Insulin.
|Components||1 x 96 tests|
|50X Washing Solution||1 x 20ml|
|Cover Foil||1 unit|
|C-Peptide HRP and Biotin Conjugate||1 x 13ml|
|C-Peptide Standard 0 - 0 ng/mL (Lyophilized)||1 vial|
|C-Peptide Standard 1 - 0.2 ng/mL (Lyophilized)||1 vial|
|C-Peptide Standard 2 - 1.0 ng/mL (Lyophilized)||1 vial|
|C-Peptide Standard 3 - 2.0 ng/mL (Lyophilized)||1 vial|
|C-Peptide Standard 4 - 5.0 ng/mL (Lyophilized)||1 vial|
|C-Peptide Standard 5 - 10.0 ng/mL (Lyophilized)||1 vial|
|Stop Solution||1 x 15ml|
|Streptavidin Coated Microplate (12 x 8 wells)||1 unit|
|Strip holder||1 unit|
|TMB Substrate Solution||1 x 15ml|
Our Abpromise guarantee covers the use of ab178641 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
ab178641 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"