Anti-C Reactive Protein antibody - Aminoterminal end (ab65842)

Overview

  • Product nameAnti-C Reactive Protein antibody - Aminoterminal end
    See all C Reactive Protein primary antibodies
  • Description
    Rabbit polyclonal to C Reactive Protein - Aminoterminal end
  • Tested applicationsSuitable for: WB, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Pig
  • Immunogen

    A synthetic peptide corresponding to a sequence at the N-terminal of human C Reactive Protein identical to the related rat and mouse sequence.

  • Positive control
    • Rat liver tissue lysate and mouse lung tissue.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab65842 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.5 µg/ml. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa).
IHC-Fr Use at an assay dependent concentration.

Target

Anti-C Reactive Protein antibody - Aminoterminal end images

  • ab65842 staining C Reactive Protein in mouse lung tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, permeabilized with 0.5% Triton X-100 and blocked for 5 minutes at 22°C. Samples were incubated with primary antibody (1/500 in 1X PBS) for 17 hours at 4°C. A Biotin-conjugated horse anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.

    See Abreview

  • Anti-C Reactive Protein antibody - Aminoterminal end (ab65842) at 2 µg/ml + rat liver tissue lysate at 30 µg

    Predicted band size : 25 kDa
    Observed band size : 25 kDa

References for Anti-C Reactive Protein antibody - Aminoterminal end (ab65842)

ab65842 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Frozen sections)
Blocking step ScyTek Super Block as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: 22°C
Sample Mouse Tissue sections (Lung)
Specification Lung
Permeabilization Yes - 0.5% Triton X-100
Fixative Paraformaldehyde
Username

Mrs. Aleksandra Kotlyarova

Verified customer

Submitted Oct 29 2014

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Pig Tissue sections (Pig Heart Tissue)
Specification Pig Heart Tissue
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10mM Sodium Citrate
Permeabilization No
Blocking step H2O2 containing sodium azide as blocking agent for 5 minute(s) · Concentration: 0.03%
Username

Tim Thayer

Verified customer

Submitted Jul 28 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"