Purification notesWhole antiserum is fractionated and chromatographed by ion exchange chromatography to provide the IgG fraction. Identity and purity of the antibody is established by immunoelectrophoresis. Electrophoresis of the antibody preparation followed by diffusion versus anti-rabbit IgG and anti-rabbit whole serum result in single arcs of precipitation in the gamma region.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesDID: Use at an assay dependent dilution. Titer: Minimum 1:8 Using an Ouchterlony double diffusion (ODD) assay, in 1% agarose, 5 ml of serially diluted reconstituted antiserum is reacted against 5 ml of 1 mg/ml solution of purified C-Reactive Protein (well separation: 7.5 mm center to center). Titer is equivalent to the highest dilution of antiserum resulting in a visible preciptate after 24 hours.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionDisplays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells.
Tissue specificityFound in plasma.
Sequence similaritiesBelongs to the pentaxin family. Contains 1 pentaxin domain.