Anti-C Reactive Protein antibody [C6] (ab8278)

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Overview

  • Product name
    Anti-C Reactive Protein antibody [C6]
    See all C Reactive Protein primary antibodies
  • Description
    Mouse monoclonal [C6] to C Reactive Protein
  • Host species
    Mouse
  • Tested applications
    Suitable for: ELISA, Sandwich ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length native protein (purified) (Human).

  • General notes
    Specific antibody titer in ELISA is > 10 to the power of -6

Properties

Applications

Our Abpromise guarantee covers the use of ab8278 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent dilution. Can be paired for ELISA with Mouse monoclonal [C2] to C Reactive Protein (HRP) (ab24461). Capture
Sandwich ELISA Use at an assay dependent dilution. Can be paired for Sandwich ELISA with Mouse monoclonal [C2] to C Reactive Protein (ab136176) and Mouse monoclonal [C5] to C Reactive Protein (ab8279). Recommended pairs for sandwich immunoassay
(capture - detection):
C2 - C6
C5 - C6
C7 - C6.

Target

  • Function
    Displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells.
  • Tissue specificity
    Found in plasma.
  • Sequence similarities
    Belongs to the pentaxin family.
    Contains 1 pentaxin domain.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
    • Alternative names
      • Pentraxin 1, short antibody
      • C reactive protein antibody
      • C reactive protein pentraxin related antibody
      • C-reactive protein(1-205) antibody
      • CRP antibody
      • CRP_HUMAN antibody
      • MGC88244 antibody
      • Pentraxin 1 antibody
      • PTX 1 antibody
      • PTX1 antibody
      see all

    References

    ab8278 has not yet been referenced specifically in any publications.

    Publishing research using ab8278? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    12 reviews or Q&A

    10
    Votes: Highest First

    Thank you for your reply. I just wonder if only one antibody binds to one CRP molecule or five antibody binds to one CRP molecule since the CRP molecule is a pentamer containing five identical monomers. Or isthis informationalso confidential?

    ...

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    Thank you for contacting us.

    I am sorry we do not have any data to confirm how many ab molecules binds to CRP. Theoretically, I agree there is possibility of binding 5 ab molecule per pentamer of CRP however we can't be sure as we do not know ...

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    Sorry that I did not include the product codes in the previous email. The product codes for anti-CRP C2 and C6 are ab24461 and ab8278, respectively.

    Thank you

    Thank you for contacting us. Dr. Tanya is out of office today so I am dealing with her inquiries.

    The immunogen for these antibodies is a proprietary information. These antibodies are raised sing full length human CRP protein. The two products...

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    Thank you for updating this. My previous order ***.

    Would you please ship it after Aug. 20? I'll not be available next week. I prefer to get it when I'm in the lab.

    Thanks,

    Thank you very much for your reply.

    It will be no problem to ship it after August 20. We are actually expecting to have this antibody in stock by August 27, so it should arrive shortly after that date. The antibody will be arriving free of ch...

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    High background using C5 (capture) and C6 (Cy5.5 conjugated detection) antibody in sELISA. Plasma samples and recombinant standards.

    Thank you again for letting us know about the trouble with the CRP antibodies in ELISA.

    We now have the unconjugated C2 clone in our catalog as ab136176, and I'd be happy to send a free of charge vial for your to try.

    http://www.abc...

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    Thank you for sharing this with me. It seems that the capturing antibody is biotinylated. That could explain the non-specific issue. It would be nice to get the un-conjugated C2 antibody to test.

    Do you have the protocol for testing blood pl...

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    Thank you very much for your reply.

    I agree that the differences in the protocol may be contributing to the background you're experiencing. I'm working with my colleagues to see if we can provide the unconjugated C2, and I will update you as ...

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    High background using C5 (capture) and C6 (Cy5.5 conjugated detection) antibody in sELISA. Plasma samples and recombinant standards.

    Thank you very much for your call earlier this week and for letting us know about the trouble with these antibodies.

    I've heard back from the lab, and these antibodies were actually tested together in a one-step fluoroimmunoassay;please see t...

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    We are currently looking for a good pairing for C-Reactive protein antibodies and wonder if you guys could recommend any pair of antibodies? The antibodies will be used in sandwich ELISA and we are looking for antibodies which are already paired by Abc...

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    Thank you for contacting us.
    We do not use the terminology C number. These does not depicts any information other the clone numbers for a particular antibody.
    The sELISA pairs we recommend are
    The best pair is C2-C6 (ab24461 detection - ...

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    Hello,   We recently purchased and tested the 500 ug (2mg/ml) ab8278 and were wondering whether or not you sell it in a more concentrated amount/larger lyophilized amount at 2mg/ml.   Thank you.

    Thank you for contacting Abcam. If you wish to place a bulk order I can happily forward your request to in house specialist how deals with these matters. However we generally only do custom batches for larger orders. If you just want to...

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    What concentrations of capture and detection antibodies were used to generate the curve on your ab8278 datasheet?

    Thank you for your enquiry. For the curve on our datasheet, the following amounts of antibody were used: C2-biotinylated: 100 ng/well (100 ul) C6-Eu (Europium) labeled: 200 ng/well (50 ul) Please do note that the amounts and concentrations depen...

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    Which form of the protein was used as the immunogen for this antibody: monomer or pentamer?

    Thank you for your enquiry. I can confirm that the antigen for anti-CRP ab8278 is the pentamer form. Please do not hesitate to contact us if you have additional questions.

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