Overview

  • Product nameC Reactive Protein (PTX1) Rat ELISA Kit
    See all C Reactive Protein kits
  • Detection methodColorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall 4.9%
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Urine, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    = 0.7 ng/ml
  • Range
    0.78 ng/ml - 50 ng/ml
  • Recovery

    96 %

  • Assay time
    3h 30m
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Rat
  • Product overview

    Abcam’s C Reactive Protein (PTX1) Rat in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of C Reactive Protein in urine, plasma, serum, and cell culture supernatants.

    A C Reactive Protein specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a C Reactive Protein specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of C Reactive Protein captured in plate.

  • Tested applicationsSandwich ELISAmore details
  • PlatformMicroplate

Properties

  • FunctionDisplays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells.
  • Tissue specificityFound in plasma.
  • Sequence similaritiesBelongs to the pentaxin family.
    Contains 1 pentaxin domain.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Alternative names
    • C reactive protein pentraxin related
    • C-reactive protein
    • C-reactive protein(1-205)
    • CRP
    • CRP_HUMAN
    • MGC149895
    • MGC88244
    • Pentraxin 1
    • Pentraxin 1 short
    • PTX 1
    • PTX1
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab108827 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

C Reactive Protein (PTX1) Rat ELISA Kit images

  • Representative Standard Curve using ab108827.

Protocols

References for C Reactive Protein (PTX1) Rat ELISA Kit (ab108827)

ab108827 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"