Anti-c-Rel (phospho S503) antibody (ab30624)

Overview

  • Product name
    Anti-c-Rel (phospho S503) antibody
    See all c-Rel primary antibodies
  • Description
    Rabbit polyclonal to c-Rel (phospho S503)
  • Specificity
    ab30624 recognises endogenous levels of c-Rel only when phosphorylated at Serine 503.
  • Tested applications
    Suitable for: WB, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic phosphopeptide derived from human c-Rel around the phosphorylation site of Serine 503.

  • Positive control
    • Breast carcinoma, MDA-MB-435 cells

Properties

Applications

Our Abpromise guarantee covers the use of ab30624 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 69 kDa.
IHC-P Use at an assay dependent concentration.
ELISA 1/1000.

Target

  • Function
    Proto-oncogene that may play a role in differentiation and lymphopoiesis. NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. The NF-kappa-B heterodimer RELA/p65-c-Rel is a transcriptional activator.
  • Sequence similarities
    Contains 1 RHD (Rel-like) domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Avian reticuloendotheliosis antibody
    • C REL antibody
    • C Rel protein antibody
    • c Rel proto oncogene protein antibody
    • Oncogene REL antibody
    • Oncogene REL avian reticuloendotheliosis antibody
    • Proto-oncogene c-Rel antibody
    • REL antibody
    • REL_HUMAN antibody
    • v rel avian reticuloendotheliosis viral oncogene homolog antibody
    • v rel reticuloendotheliosis viral oncogene homolog antibody
    • V rel reticuloendotheliosis viral oncogene homolog (avian) antibody
    see all

Images

  • All lanes : Anti-c-Rel (phospho S503) antibody (ab30624) at 1/500 dilution

    Lane 1 : extracts from MDA-MB-435 cells (5-30ug).
    Lane 2 : extracts from MDA-MB-435 cells (5-30ug), preincubated with synthesized peptide (negative control).


    Predicted band size : 69 kDa
    Observed band size : 60 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemical analysis of paraffin embedded breast carcinoma tissue sections, using 1/50 c-Rel (Phospho- Ser503) Antibody (ab30624). Left: untreated sample; Right: sample preincubated with synthesized phosphopeptide (negative control).

References

ab30624 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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