Recombinant
RabMAb

Anti-C10orf35 antibody [EPR14950] (ab187150)

Overview

  • Product name
    Anti-C10orf35 antibody [EPR14950]
    See all C10orf35 primary antibodies
  • Description
    Rabbit monoclonal [EPR14950] to C10orf35
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Recombinant fragment within Human C10orf35 aa 1-100. The exact sequence is proprietary.
    Database link: Q96D05

  • Positive control
    • Human fetal brain, Rat brain and Rat kidney tissue lysates; MCF-7, A431 and PC-12 cell lysates.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    EPR14950
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab187150 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 13 kDa (predicted molecular weight: 13 kDa).
IP 1/30 - 1/50.

Target

Images

  • All lanes : Anti-C10orf35 antibody [EPR14950] (ab187150) at 1/2000 dilution

    Lane 1 : Human fetal brain tissue lysate
    Lane 2 : MCF-7 cell lysate
    Lane 3 : A431 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 13 kDa



    Blocking and dilution buffer: 5% NFDM/TBST

  • All lanes : Anti-C10orf35 antibody [EPR14950] (ab187150) at 1/2000 dilution

    Lane 1 : Rat brain tissue lysate
    Lane 2 : Rat kidney tissue lysate
    Lane 3 : PC-12 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 13 kDa



    Blocking and dilution buffer: 5% NFDM/TBST

  • Immunoprecipitation analysis of human fetal brain tissue lysate labeling C10orf35 using ab187150 at 1/50 dilution. A Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 was used as secondary antibody.

References

ab187150 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab187150.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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