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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide Constituents: 59% PBS, 40% Glycerol
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Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/100 - 1/250.
Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Anti-C10orf53 antibody images
Immunocytochemistry/ Immunofluorescence - Anti-C10orf53 antibody (ab122426)
Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus but not nucleoli and cytoplasm. Recommended concentration of ab122426 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Western blot - Anti-C10orf53 antibody (ab122426)
Lane 1 : Lanes 2 - 3 : Anti-C10orf53 antibody (ab122426) Lane 1 : Marker Lane 2 : HEK293T cell lysate - vector only transfected Lane 3 : HEK293T cell lysate - overexpressing C10orf53 with a C-terminal myc-DDK tag (~3.1kDa)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C10orf53 antibody (ab122426)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testis tissue labelling C10orf53 with ab122426. Staining shows moderate cytoplasmic positivity in subset of cells in seminiferous ducts.
References for Anti-C10orf53 antibody (ab122426)
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"