Overview

  • Product name
    Anti-C10orf90 antibody
  • Description
    Rabbit polyclonal to C10orf90
  • Tested applications
    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence, corresponding to amino acids 169-262 (AEDTLFQAPP ALANGAHPGR HQRSFACTEF SRNSSVVRLK VPEAHTGLCE RRKYWVTHAD DKETSFSPDT PLSGKSPLVF SSCVHLRVSQ QCPD) of Human C10orf90.

  • Positive control
    • Human kidney tissue; RT-4 and U-251 MG cell lysate; Human Liver and Tonsil tissue lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab122497 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500.

Target

Images

  • Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus but not nucleoli, plasma membrane and cytoplasm. Recommended concentration of ab122497 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab122497 at 1/425 staining C10orf90 in Paraffin Embedded Human kidney tissue.
  • All lanes : Anti-C10orf90 antibody (ab122497) at 1/250 dilution

    Lane 1 : RT-4 lysate
    Lane 2 : U-251 MG lysate
    Lane 3 : Human Plasma
    Lane 4 : Human Liver lysate
    Lane 5 : Human Tonsil lysate

    Developed using the ECL technique

References

This product has been referenced in:
  • Song F  et al. A functional genetic variant in fragile-site gene FATS modulates the risk of breast cancer in triparous women. BMC Cancer 15:559 (2015). IHC . Read more (PubMed: 26223354) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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