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Rabbit polyclonal to C10orf90
antigen sequence, corresponding to amino acids 169-262 (AEDTLFQAPP ALANGAHPGR HQRSFACTEF SRNSSVVRLK VPEAHTGLCE RRKYWVTHAD DKETSFSPDT PLSGKSPLVF SSCVHLRVSQ QCPD) of Human C10orf90.
Human kidney tissue; RT-4 and U-251 MG cell lysate; Human Liver and Tonsil tissue lysate.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
1/250 - 1/500.
Information by UniProt
Chromosome 10 open reading frame 90 antibody
Immunocytochemistry/ Immunofluorescence - Anti-C10orf90 antibody (ab122497)
Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus but not nucleoli, plasma membrane and cytoplasm. Recommended concentration of ab122497 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C10orf90 antibody (ab122497)
ab122497 at 1/425 staining C10orf90 in Paraffin Embedded Human kidney tissue.
Western blot - Anti-C10orf90 antibody (ab122497)
All lanes : Anti-C10orf90 antibody (ab122497) at 1/250 dilution Lane 1 : RT-4 lysate Lane 2 : U-251 MG lysate Lane 3 : Human Plasma Lane 4 : Human Liver lysate Lane 5 : Human Tonsil lysate Developed using the ECL technique.
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