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antigen sequence: MWSFLPGAES VSMGPVPGVS SLGACWTHDQ DSGRAEDRPQ APRITQYTWV LSFLFTEKPQ TRSTSPISHQ GQ, corresponding to amino acids 1-72 of Human C10orf91 (Q5T1B1).
Our Abpromise guarantee covers the use of ab122382 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 - 4 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100.
|IHC-P||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
Immunocytochemistry/Immunofluorescence analysis of A549 cells labelling C10orf91 with ab122382 shows positivity in the nucleus but is excluded from the nucleoli.
ab122382 has not yet been referenced specifically in any publications.