Overview

  • Product name
    Anti-C10orf93 antibody
  • Description
    Rabbit polyclonal to C10orf93
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 1-206 of Human C10orf93, Isoform 2 (AAH44661).

  • Positive control
    • A549, HeLa and HCT116 cells and whole cell lysates; HBL435 xenograft tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 20% Glycerol, 0.75% Glycine
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab111682 is purified by antigen-affinity chromatography.
  • Clonality
    Polyclonal
  • Isotype
    IgG

Applications

Our Abpromise guarantee covers the use of ab111682 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 29 kDa.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/500.

Target

Images

  • Anti-C10orf93 antibody (ab111682) at 1/1000 dilution + HCT116 whole cell lysate at 30 µg

    Predicted band size: 29 kDa



    12% SDS-PAGE.
  • ab111682 at 1/200 dilution staining C10orf93 in methanol-fixed HeLa cells by Immunofluorescence. Lower image shows cells co-stained with Hoechst 33342.
  • ab111682 at 1/500 dilution staining C10orf93 in paraffin-embedded HBL435 xenograft tissue by Immunohistochemistry.

References

ab111682 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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