Overview

  • Product name
    Anti-C11B2 antibody [EPR10494]
    See all C11B2 primary antibodies
  • Description
    Rabbit monoclonal [EPR10494] to C11B2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
    Unsuitable for: ICC
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human C11B2 aa 1-100.

  • Positive control
    • Human adrenal gland lysate; Human kidney and liver tissues.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab168388 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 58 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for ICC.
  • Target

    • Function
      Preferentially catalyzes the conversion of 11-deoxycorticosterone to aldosterone via corticosterone and 18-hydroxycorticosterone.
    • Involvement in disease
      Defects in CYP11B2 are the cause of corticosterone methyloxidase type 1 deficiency (CMO-1 deficiency) [MIM:203400]; also known as aldosterone deficiency due to defect in 18-hydroxylase or aldosterone deficiency I. CMO-1 deficiency is an autosomal recessive disorder of aldosterone biosynthesis. There are two biochemically different forms of selective aldosterone deficiency be termed corticosterone methyloxidase (CMO) deficiency type 1 and type 2. In CMO-1 deficiency, aldosterone is undetectable in plasma, while its immediate precursor, 18-hydroxycorticosterone, is low or normal.
      Defects in CYP11B2 are the cause of corticosterone methyloxidase type 2 deficiency (CMO-2 deficiency) [MIM:610600]. CMO-2 is an autosomal recessive disorder of aldosterone biosynthesis. In CMO-2 deficiency, aldosterone can be low or normal, but at the expense of increased secretion of 18-hydroxycorticosterone. Consequently, patients have a greatly increased ratio of 18-hydroxycorticosterone to aldosterone and a low ratio of corticosterone to 18-hydroxycorticosterone in serum.
      Defects in CYP11B2 are a cause of familial hyperaldosteronism type 1 (FH1) [MIM:103900]. It is a disorder characterized by hypertension, variable hyperaldosteronism, and abnormal adrenal steroid production, including 18-oxocortisol and 18-hydroxycortisol. There is significant phenotypic heterogeneity, and some individuals never develop hypertension. Note=The molecular defect causing hyperaldosteronism familial type 1 is an anti-Lepore-type fusion of the CYP11B1 and CYP11B2 genes. The hybrid gene has the promoting part of CYP11B1, ACTH-sensitive, and the coding part of CYP11B2.
    • Sequence similarities
      Belongs to the cytochrome P450 family.
    • Cellular localization
      Mitochondrion membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • ALDOS antibody
      • Aldosterone synthase antibody
      • Aldosterone-synthesizing enzyme antibody
      • C11B2_HUMAN antibody
      • CYP11B2 antibody
      • CYPXIB2 antibody
      • Cytochrome P-450Aldo antibody
      • Cytochrome P-450C18 antibody
      • Cytochrome P450 11B2 antibody
      • Cytochrome P450 11B2, mitochondrial antibody
      • mitochondrial antibody
      • P-450Aldo antibody
      • P-450C18 antibody
      • Steroid 18-hydroxylase antibody
      see all

    Images

    • ab168388 showing +ve staining in Human heart muscle tissue.

    • ab168388 showing +ve staining in Human normal pancreas tissue.

    • Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling C11B2 with ab168388 at 1/100 dilution.
    • Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling C11B2 with ab168388 at 1/100 dilution.
    • Anti-C11B2 antibody [EPR10494] (ab168388) at 1/1000 dilution + Human adrenal gland lysate at 10 µg

      Secondary
      Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 58 kDa

    References

    This product has been referenced in:
    • Mopidevi B  et al. Variable transcriptional regulation of the human aldosterone synthase gene causes salt-dependent high blood pressure in transgenic mice. Circ Cardiovasc Genet 8:30-9 (2015). Read more (PubMed: 25504670) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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