Recombinant Anti-C11B2/CYP11B2 antibody [EPR10495] (ab167413)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10495] to C11B2/CYP11B2
- Suitable for: WB, IP
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-C11B2/CYP11B2 antibody [EPR10495]
See all C11B2/CYP11B2 primary antibodies -
Description
Rabbit monoclonal [EPR10495] to C11B2/CYP11B2 -
Host species
Rabbit -
Specificity
The immunogen used for this product shares 5 consecutive identical amino acids with family members like CYP11B1, CYP3A4, CYP3A43, CYP3A5, CYP3A7. Cross-reactivity with these proteins have not been confirmed experimentally.
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Tested applications
Suitable for: WB, IPmore details
Unsuitable for: Flow Cyt or ICC/IF -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human C11B2/CYP11B2 aa 300-400. The exact sequence is proprietary.
Database link: P19099 -
Positive control
- IP: MCF7 cell lysates WB: Human adrenal gland, NIH:OVCAR-3 and MCF7 cell lysates, human tissue lysates;
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR10495 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab167413 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 58 kDa.
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IP |
1/10 - 1/100.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 58 kDa. |
IP
1/10 - 1/100. |
Target
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Function
Preferentially catalyzes the conversion of 11-deoxycorticosterone to aldosterone via corticosterone and 18-hydroxycorticosterone. -
Involvement in disease
Defects in CYP11B2 are the cause of corticosterone methyloxidase type 1 deficiency (CMO-1 deficiency) [MIM:203400]; also known as aldosterone deficiency due to defect in 18-hydroxylase or aldosterone deficiency I. CMO-1 deficiency is an autosomal recessive disorder of aldosterone biosynthesis. There are two biochemically different forms of selective aldosterone deficiency be termed corticosterone methyloxidase (CMO) deficiency type 1 and type 2. In CMO-1 deficiency, aldosterone is undetectable in plasma, while its immediate precursor, 18-hydroxycorticosterone, is low or normal.
Defects in CYP11B2 are the cause of corticosterone methyloxidase type 2 deficiency (CMO-2 deficiency) [MIM:610600]. CMO-2 is an autosomal recessive disorder of aldosterone biosynthesis. In CMO-2 deficiency, aldosterone can be low or normal, but at the expense of increased secretion of 18-hydroxycorticosterone. Consequently, patients have a greatly increased ratio of 18-hydroxycorticosterone to aldosterone and a low ratio of corticosterone to 18-hydroxycorticosterone in serum.
Defects in CYP11B2 are a cause of familial hyperaldosteronism type 1 (FH1) [MIM:103900]. It is a disorder characterized by hypertension, variable hyperaldosteronism, and abnormal adrenal steroid production, including 18-oxocortisol and 18-hydroxycortisol. There is significant phenotypic heterogeneity, and some individuals never develop hypertension. Note=The molecular defect causing hyperaldosteronism familial type 1 is an anti-Lepore-type fusion of the CYP11B1 and CYP11B2 genes. The hybrid gene has the promoting part of CYP11B1, ACTH-sensitive, and the coding part of CYP11B2. -
Sequence similarities
Belongs to the cytochrome P450 family. -
Cellular localization
Mitochondrion membrane. - Information by UniProt
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Database links
- Entrez Gene: 1585 Human
- Omim: 124080 Human
- SwissProt: P19099 Human
- Unigene: 632054 Human
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Alternative names
- ALDOS antibody
- Aldosterone synthase antibody
- Aldosterone-synthesizing enzyme antibody
see all
Images
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All lanes : Anti-C11B2/CYP11B2 antibody [EPR10495] (ab167413) at 1/1000 dilution
Lane 1 : Human adrenal gland tissue lysate
Lane 2 : Human kidney tissue lysate
Lane 3 : Human heart tissue lysate
Lane 4 : Human lung tissue lysate
Lane 5 : Human liver tissue lysate
Lane 6 : HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate
Lane 7 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 58 kDa
Observed band size: 48 kDa why is the actual band size different from the predicted?
Exposure time: 20 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST
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All lanes : Anti-C11B2/CYP11B2 antibody [EPR10495] (ab167413) at 1/1000 dilution
Lane 1 : MCF-7 cell lysate with Human C11B2/CYP11B2 peptide
Lane 2 : MCF-7 cell lysate with Human C11B1/CYP11B2 peptide
Lane 3 : MCF-7 cell lysate with no blocking peptide
Predicted band size: 58 kDa
Observed band size: 48 kDa why is the actual band size different from the predicted?
Exposure time: 3 seconds -
All lanes : Anti-C11B2/CYP11B2 antibody [EPR10495] (ab167413) at 1/1000 dilution
Lane 1 : Human adrenal gland tissue lysate
Lane 2 : NIH:OVCAR-3 cell lysate
Lane 3 : MCF7 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution
Predicted band size: 58 kDa -
Detection of C11B2/CYP11B2 by Western Blot of Immunprecipitate. MCF7 cell lysate immunoprecipitated using ab167413 at 1/10 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab167413 has been referenced in 3 publications.
- Stelcer E et al. Adropin Stimulates Proliferation and Inhibits Adrenocortical Steroidogenesis in the Human Adrenal Carcinoma (HAC15) Cell Line. Front Endocrinol (Lausanne) 11:561370 (2020). PubMed: 33133015
- Zhang G et al. MiR-193a-3p functions as a tumour suppressor in human aldosterone-producing adrenocortical adenoma by down-regulating CYP11B2. Int J Exp Pathol 99:77-86 (2018). PubMed: 29665181
- França MM et al. POD-1/Tcf21 overexpression reduces endogenous SF-1 and StAR expression in rat adrenal cells. Braz J Med Biol Res 48:1087-94 (2015). WB . PubMed: 26421867