Overview

  • Product name
  • Description
    Rabbit polyclonal to C11orf1
  • Tested applications
    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence: QERYDLRNIV QPKPLPSQFG HYFETTYDTS YNNKMPLSTH RFKREPHWFP GHQPELDPPR YKCTEKSTYM NSYSKP, corresponding to C terminal amino acids 75-150 of Human C11orf1.

  • Positive control
    • Human hippocampus tissue; C11orf1 over-expression HEK293T lysate; U 251 MG cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab122472 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500.
ICC/IF Use a concentration of 1 - 4 µg/ml. Fixation/Permeabilization: PFA/Triton X-100

Target

Anti-C11orf1 antibody images

  • ab122472, at 1/200, staining C11orf1 in paraffin-embedded Human hippocampus tissue by Immunohistochemistry.
  • All lanes : Anti-C11orf1 antibody (ab122472) at 1/250 dilution

    Lane 1 : Negative control (vector only transfected HEK293T lysate)
    Lane 2 : Over-expression lysate (co-expressed with a C-terminal myc-DDK tag [~3.1 kDa] in HEK293T cells)

    Developed using the ECL technique
  • ab122472, at 4 µg/ml, staining C11orf1 in in PFA/Triton X-100 fixed/permeabilized U 251 MG cells by Immunofluorescence (green).

References for Anti-C11orf1 antibody (ab122472)

ab122472 has not yet been referenced specifically in any publications.

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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