Overview

  • Product nameAnti-C11ORF54 antibody
    See all C11ORF54 primary antibodies
  • Description
    Rabbit polyclonal to C11ORF54
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide: VDCPDLTKEP FTFPVKGICG KTRIAEVGGV PYLLPLVNQK KVYDLNKIAK, corresponding to amino acids 36-85 of Human C11ORF54 (NP_054758).

  • Positive control
    • Fetal muscle lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab80321 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 35 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1/312500.

Target

  • RelevanceC11ORF54 exhibits ester hydrolase activity on the substrate p-nitrophenyl acetate.
  • Cellular localizationNuclear
  • Database links
  • Alternative names
    • Chromosome 11 open reading frame 54 antibody
    • Ester hydrolase C11orf54 antibody
    • Hypothetical protein LOC28970 antibody
    • LP4947 antibody
    • PTD012 antibody
    see all

Anti-C11ORF54 antibody images

  • Anti-C11ORF54 antibody (ab80321) at 1 µg/ml (in 5% skim milk / PBS buffer) + fetal muscle lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 35 kDa
    Observed band size : 32 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 65 kDa. We are unsure as to the identity of these extra bands.

References for Anti-C11ORF54 antibody (ab80321)

ab80321 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"