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Rabbit polyclonal to C11orf96
antigen sequence, corresponding to amino acids 362-429 (RQSRFKTQPV TFDEIQEVEE EGVSPMEEEK AKKSFLQSLE CLRRSTQSLS LQREQLSSCK LRNSLDSS) of Human C11orf96.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.02% Sodium azide Constituents: 59% PBS, 40% Glycerol
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Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Phosphorylated upon DNA damage, probably by ATM or ATR.
Information by UniProt
Immunocytochemistry/ Immunofluorescence - Anti-C11orf96 antibody (ab122512)
Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus but not nucleoli, golgi apparatus and focal adhesions. Recommended concentration of ab122512 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C11orf96 antibody (ab122512)
ab122512 at 1/450 dilution staining C11orf96 in Paraffin-embedded Human Prostate tissue by Immunohistochemistry.
has not yet been referenced specifically in any publications.
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