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Rabbit polyclonal to C12orf35
antigen, corresponding to amino acids 1597-1729 of Human C12orf35 (Q9HCM1).
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
Immunogen affinity purified
Our Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
||1/10 - 1/20. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. , in retrieval buffer pH 6.
Information by UniProt
- C12orf35 antibody
- CL035_HUMAN antibody
- Uncharacterized protein C12orf35 antibody
Immunocytochemistry/ Immunofluorescence - Anti-C12orf35 antibody (ab121408)
Immunofluorescent staining of Human cell line U-251MG shows positivity in nucleoli. Recommended concentration of ab121408 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C12orf35 antibody (ab121408)
Immunohistochemical staining of Human cerebellum shows distinct cytoplasmic positivity in cerebellar tissue, using 1/10 ab121408.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"