Anti-C12orf48 antibody - C-terminal (ab107308)

Overview

  • Product nameAnti-C12orf48 antibody - C-terminal
    See all C12orf48 primary antibodies
  • Description
    Rabbit polyclonal to C12orf48 - C-terminal
  • Tested applicationsSuitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH, between amino acids 529-558 from the C terminal region of Human C12orf48 (NP_060385.3).

  • Positive control
    • A549 cell line lysate. This antibody gave a positive result when used in the following methanol fixed cell line: HeLa.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab107308 is purified through a protein A column, followed by peptide affinity purification.
  • ClonalityPolyclonal
  • IsotypeIgG

Applications

Our Abpromise guarantee covers the use of ab107308 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB 1/100 - 1/500. Predicted molecular weight: 65 kDa.

Target

  • FunctionMay be involved in RNA metabolism. Binds single-strand DNA and poly(A) homopolymers (By similarity). Positively regulate the poly(ADP-ribosyl)ation activity of PARP1.
  • Tissue specificityRestricted to testis. Overexpressed in multiple cancer cells.
  • Sequence similaritiesBelongs to the PARP1BP family.
  • Cellular localizationCytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • AROM antibody
    • C12orf48 antibody
    • Chromosome 12 open reading frame 48 antibody
    • FLJ20641 antibody
    • PARP 1 binding protein antibody
    • PARP-1 binding protein antibody
    • PARP1 binding protein antibody
    • PARP1-binding protein antibody
    • PARPBP antibody
    • PR1BP_HUMAN antibody
    see all

Anti-C12orf48 antibody - C-terminal images

  • ICC/IF image of ab107308 stained HeLa cells.  The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab107308 at 5µg/mL overnight at +4°C. The secondary antibody (green) was DyLight® 488 Goat anti-Rabbit IgG (H+L) (ab96899) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-C12orf48 antibody - C-terminal (ab107308) at 1/100 dilution + A549 cell line lysate at 35 µg

    Predicted band size : 65 kDa

References for Anti-C12orf48 antibody - C-terminal (ab107308)

ab107308 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"