Overview

  • Product name
    Anti-C12orf49 antibody
  • Description
    Rabbit polyclonal to C12orf49
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to a region within the C terminal amino acids 143-192 (LERFLNRAAV AFQNLFMAVE DHFELCLAKC RTSSQSVQHE NTYRDPIAKY) of Human C12orf49 (NP_079014).

  • Positive control
    • HeLa cell lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab82682 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 24 kDa (predicted molecular weight: 24 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay 1/312500.

Target

  • Relevance
    C12orf49 belongs to the UPF0454 family. The exact function of C12orf49 remains unknown.
  • Cellular localization
    Secreted
  • Database links
  • Alternative names
    • Chromosome 12 open reading frame 49 antibody
    • Hypothetical protein LOC79794 antibody
    • UPF0454 protein C12orf49 homolog antibody

Images

  • Anti-C12orf49 antibody (ab82682) at 1 µg/ml (5% skim milk / PBS buffer) + HeLa cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 24 kDa
    Observed band size : 24 kDa

References

ab82682 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab82682.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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