Overview

  • Product nameAnti-C12orf65 antibody
    See all C12orf65 primary antibodies
  • Description
    Rabbit polyclonal to C12orf65
  • Tested applicationsSuitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant antigen sequence: GLFHFPTPLT RICPAPWGLR LWEKLTLLSP GIAVTPVQMA GKKDYPALLS LDENELEEQF VKGHGPGGQA TNKTSNC, corresponding to amino acids 5-81 of Human C12orf65

  • Positive control
    • Human urinary bladder tissue (IHC-P); overexpress HEK293T cell lysate (WB)

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab122448 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500.

Target

  • FunctionMay act as a codon-independent translation release factor that has lost all stop codon specificity and directs the termination of translation in mitochondrion.
  • Involvement in diseaseDefects in C12orf65 are the cause of combined oxidative phosphorylation deficiency type 7 (COXPD7) [MIM:613559]. A mitochondrial disease resulting in encephalomyopathy. Clinical manifestations include psychomotor delay and regression, ataxia, optic atrophy, nystagmus and muscle atrophy and weakness.
  • Sequence similaritiesBelongs to the prokaryotic/mitochondrial release factor family.
  • Cellular localizationMitochondrion.
  • Information by UniProt
  • Database links
  • Alternative names
    • C12orf65 antibody
    • chromosome 12 open reading frame 65 antibody
    • CL065_HUMAN antibody
    • COXPD7 antibody
    • mitochondrial antibody
    • Probable peptide chain release factor C12orf65 antibody
    • probable peptide chain release factor C12orf65, mitochondrial antibody
    • SPG55 antibody
    see all

Anti-C12orf65 antibody images

  • ab122448, at 1/200 dilution, staining C12orf65 in paraffin-embedded Human urinary bladder tissue by Immunohistochemistry.
  • All lanes : Anti-C12orf65 antibody (ab122448) at 1/250 dilution

    Lane 1 : Negative control (vector only transfected HEK293T lysate)
    Lane 2 : Over expression lysate: coexpressed with a C terminal myc DDK tag (~3.1 kDa) in mammalian HEK293T cells

References for Anti-C12orf65 antibody (ab122448)

ab122448 has not yet been referenced specifically in any publications.

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