Overview

  • Product name
  • Description
    Mouse polyclonal to C13orf8
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length protein corresponding to amino acids 1-812 of Human C13orf8 (NP_115812.1).

  • Positive control
    • C13orf8 transfected 293T cell line; HeLa cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    pH: 7.20
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab169249 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 89 kDa.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • Function
    Required for proper alignment of chromosomes at metaphase and their accurate segregation during mitosis. Involved in the maintenance of spindle microtubules attachment to the kinetochore during sister chromatid biorientation. May recruit CENPE and CENPF to the kinetochore.
  • Sequence similarities
    Contains 1 C2H2-type zinc finger.
  • Post-translational
    modifications
    Phosphorylated by CDK1. Mitotic phosphorylation is required for the attachment of spindle microtubules to the kinetochore.
  • Cellular localization
    Nucleus. Chromosome. Chromosome > centromere > kinetochore. Cytoplasm > cytoskeleton > spindle.
  • Information by UniProt
  • Database links
  • Alternative names
    • C13orf8 antibody
    • CAMP antibody
    • CHAMP antibody
    • CHAMP1 antibody
    • CHAP1_HUMAN antibody
    • chromosome 13 open reading frame 8 antibody
    • Chromosome alignment-maintaining phosphoprotein 1 antibody
    • FLJ90413 antibody
    • KIAA1802 antibody
    • Zinc finger protein 828 antibody
    • Zinc finger protein KIAA1802 antibody
    • ZNF828 antibody
    see all

Images

  • All lanes : Anti-C13orf8 antibody (ab169249) at 1 µg/ml

    Lane 1 : C13orf8 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 15 µl per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution
    Developed using the ECL technique

    Predicted band size : 89 kDa
  • Immunofluorescent analysis of HeLa cells labeling C13orf8 with ab169249 at 10 µg/ml.

References

ab169249 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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